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@ARTICLE{Smidt:172187,
      author       = {Smidt, Hauke and Dungait, Jennifer A. J. and Bol, Roland
                      and Selsted, Merete B. and Ambus, Per and Michelsen, Anders
                      and Smidt, Hauke},
      title        = {{B}acteria and {F}ungi {R}espond {D}ifferently to
                      {M}ultifactorial {C}limate {C}hange in a {T}emperate
                      {H}eathland, {T}raced with 13{C}-{G}lycine and {FACE} {CO}2},
      journal      = {PLoS one},
      volume       = {9},
      number       = {1},
      issn         = {1932-6203},
      address      = {Lawrence, Kan.},
      publisher    = {PLoS},
      reportid     = {FZJ-2014-05684},
      pages        = {e85070 -},
      year         = {2014},
      abstract     = {It is vital to understand responses of soil microorganisms
                      to predicted climate changes, as these directly control soil
                      carbon (C) dynamics. The rate of turnover of soil organic
                      carbon is mediated by soil microorganisms whose activity may
                      be affected by climate change. After one year of
                      multifactorial climate change treatments, at an undisturbed
                      temperate heathland, soil microbial community dynamics were
                      investigated by injection of a very small concentration
                      (5.12 µg C g−1 soil) of 13C-labeled glycine (13C2, 99
                      atom $\%)$ to soils in situ. Plots were treated with
                      elevated temperature (+1°C, T), summer drought (D) and
                      elevated atmospheric carbon dioxide (510 ppm [CO2]), as well
                      as combined treatments (TD, TCO2, DCO2 and TDCO2). The 13C
                      enrichment of respired CO2 and of phospholipid fatty acids
                      (PLFAs) was determined after 24 h. 13C-glycine incorporation
                      into the biomarker PLFAs for specific microbial groups (Gram
                      positive bacteria, Gram negative bacteria, actinobacteria
                      and fungi) was quantified using gas
                      chromatography-combustion-stable isotope ratio mass
                      spectrometry (GC-C-IRMS).Gram positive bacteria
                      opportunistically utilized the freshly added glycine
                      substrate, i.e. incorporated 13C in all treatments, whereas
                      fungi had minor or no glycine derived 13C-enrichment, hence
                      slowly reacting to a new substrate. The effects of elevated
                      CO2 did suggest increased direct incorporation of glycine in
                      microbial biomass, in particular in G+ bacteria, in an
                      ecosystem subjected to elevated CO2. Warming decreased the
                      concentration of PLFAs in general. The FACE CO2 was
                      13C-depleted (δ13C = 12.2‰) compared to ambient (δ13C =
                      ~−8‰), and this enabled observation of the integrated
                      longer term responses of soil microorganisms to the FACE
                      over one year. All together, the bacterial (and not fungal)
                      utilization of glycine indicates substrate preference and
                      resource partitioning in the microbial community, and
                      therefore suggests a diversified response pattern to future
                      changes in substrate availability and climatic factors.},
      cin          = {IBG-3},
      ddc          = {500},
      cid          = {I:(DE-Juel1)IBG-3-20101118},
      pnm          = {246 - Modelling and Monitoring Terrestrial Systems: Methods
                      and Technologies (POF2-246) / 255 - Terrestrial Systems:
                      From Observation to Prediction (POF3-255)},
      pid          = {G:(DE-HGF)POF2-246 / G:(DE-HGF)POF3-255},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000330235100053},
      pubmed       = {pmid:24454793},
      doi          = {10.1371/journal.pone.0085070},
      url          = {https://juser.fz-juelich.de/record/172187},
}