Journal Article

FZJ-2014-05684

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Bacteria and Fungi Respond Differently to Multifactorial Climate Change in a Temperate Heathland, Traced with 13C-Glycine and FACE CO2

Smidt, H. (Corresponding Author) ; Dungait, J. A. J. ; Bol, R.FZJ* ; Selsted, M. B. ; Ambus, P. ; Michelsen, A. ; Smidt, H.

2014
PLoS Lawrence, Kan.

This record in other databases:      

Please use a persistent id in citations: http://hdl.handle.net/2128/9137  doi:10.1371/journal.pone.0085070

Abstract: It is vital to understand responses of soil microorganisms to predicted climate changes, as these directly control soil carbon (C) dynamics. The rate of turnover of soil organic carbon is mediated by soil microorganisms whose activity may be affected by climate change. After one year of multifactorial climate change treatments, at an undisturbed temperate heathland, soil microbial community dynamics were investigated by injection of a very small concentration (5.12 µg C g−1 soil) of 13C-labeled glycine (13C2, 99 atom %) to soils in situ. Plots were treated with elevated temperature (+1°C, T), summer drought (D) and elevated atmospheric carbon dioxide (510 ppm [CO2]), as well as combined treatments (TD, TCO2, DCO2 and TDCO2). The 13C enrichment of respired CO2 and of phospholipid fatty acids (PLFAs) was determined after 24 h. 13C-glycine incorporation into the biomarker PLFAs for specific microbial groups (Gram positive bacteria, Gram negative bacteria, actinobacteria and fungi) was quantified using gas chromatography-combustion-stable isotope ratio mass spectrometry (GC-C-IRMS).Gram positive bacteria opportunistically utilized the freshly added glycine substrate, i.e. incorporated 13C in all treatments, whereas fungi had minor or no glycine derived 13C-enrichment, hence slowly reacting to a new substrate. The effects of elevated CO2 did suggest increased direct incorporation of glycine in microbial biomass, in particular in G+ bacteria, in an ecosystem subjected to elevated CO2. Warming decreased the concentration of PLFAs in general. The FACE CO2 was 13C-depleted (δ13C = 12.2‰) compared to ambient (δ13C = ~−8‰), and this enabled observation of the integrated longer term responses of soil microorganisms to the FACE over one year. All together, the bacterial (and not fungal) utilization of glycine indicates substrate preference and resource partitioning in the microbial community, and therefore suggests a diversified response pattern to future changes in substrate availability and climatic factors.

---

Contributing Institute(s):

1. Agrosphäre (IBG-3)

Research Program(s):

1. 246 - Modelling and Monitoring Terrestrial Systems: Methods and Technologies (POF2-246) (POF2-246)
2. 255 - Terrestrial Systems: From Observation to Prediction (POF3-255) (POF3-255)

Appears in the scientific report 2014

---

Database coverage:
; ; ; ; BIOSIS Previews ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

---