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| Home > Publications database > Quantitative images of metals in plant tissues measured by laser ablation inductively coupled plasma mass spectrometry |
| Journal Article | PreJuSER-1758 |
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2008
Amsterdam [u.a.]
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Please use a persistent id in citations: doi:10.1016/j.sab.2008.08.001
Abstract: Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was used for quantitative imaging of toxic and essential elements in thin sections (thickness of 30 or 40 pm) of tobacco plant tissues. Two-dimensional images of Mg, Fe, Mn, Zn, Cu, Cd, Rh, Pt and Pb in leaves, shoots and roots of tobacco were produced. Sections of the plant tissues (fixed onto glass slides) were scanned by a focused beam of a Nd:YAG laser in a laser ablation chamber. The ablated material was transported with argon as carrier gas to the ICP ion source at a quadrupole ICP-MS instrument. Ion intensities of the investigated elements were measured C-13(+), S-33(+) and S-34(+) together with and within the entire plant tissue section. Matrix matching standards (prepared using powder of dried tobacco leaves) were used to constitute calibration curves, whereas the regression coefficient of the attained calibration curves was typically 0.99. The variability of LA-ICP-MS process, sample heterogeneity and water content in the sample were corrected by using C-13(+) as internal standard. Quantitative imaging of the selected elements revealed their inhomogeneous distribution in leaves, shoots and roots. (C) 2008 Elsevier B.V. All rights reserved.
Keyword(s): J ; Tobacco (auto) ; Plant tissue (auto) ; Laser ablation inductively coupled plasma (auto) ; mass spectrometry (auto) ; Quantitative imaging of elements (auto)
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