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@ARTICLE{Schellenberg:187633,
      author       = {Schellenberg, Anne and Joussen, Sylvia and Moser, Kristin
                      and Hampe, Nico and Hersch, Nils and Hemeda, Hatim and
                      Schnitker, Jan and Denecke, Bernd and Lin, Qiong and Pallua,
                      Norbert and Zenke, Martin and Merkel, Rudolf and Hoffmann,
                      Bernd and Wagner, Wolfgang},
      title        = {{M}atrix elasticity, replicative senescence and {DNA}
                      methylation patterns of mesenchymal stem cells},
      journal      = {Biomaterials},
      volume       = {35},
      number       = {24},
      issn         = {0142-9612},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2015-01259},
      pages        = {6351-6358},
      year         = {2014},
      abstract     = {Matrix elasticity guides differentiation of mesenchymal
                      stem cells (MSCs) but it is unclear if these effects are
                      only transient – while the cells reside on the substrate
                      – or if they reflect persistent lineage commitment. In
                      this study, MSCs were continuously culture-expanded in
                      parallel either on tissue culture plastic (TCP) or on
                      polydimethylsiloxane (PDMS) gels of different elasticity to
                      compare impact on replicative senescence, in vitro
                      differentiation, gene expression, and DNA methylation (DNAm)
                      profiles. The maximal number of cumulative population
                      doublings was not affected by matrix elasticity.
                      Differentiation towards adipogenic and osteogenic lineage
                      was increased on soft and rigid biomaterials, respectively
                      – but this propensity was no more evident if cells were
                      transferred to TCP. Global gene expression profiles and DNAm
                      profiles revealed relatively few differences in MSCs
                      cultured on soft or rigid matrices. Furthermore, only
                      moderate DNAm changes were observed upon culture on very
                      soft hydrogels of human platelet lysate. Our results support
                      the notion that matrix elasticity influences cellular
                      behavior while the cells reside on the substrate, but it
                      does not have major impact on cell-intrinsic lineage
                      determination, replicative senescence or DNAm patterns.},
      cin          = {ICS-8 / ICS-7},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-8-20110106 / I:(DE-Juel1)ICS-7-20110106},
      pnm          = {453 - Physics of the Cell (POF2-453)},
      pid          = {G:(DE-HGF)POF2-453},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000338804500020},
      pubmed       = {pmid:24824582},
      doi          = {10.1016/j.biomaterials.2014.04.079},
      url          = {https://juser.fz-juelich.de/record/187633},
}