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@ARTICLE{Schellenberg:187633,
author = {Schellenberg, Anne and Joussen, Sylvia and Moser, Kristin
and Hampe, Nico and Hersch, Nils and Hemeda, Hatim and
Schnitker, Jan and Denecke, Bernd and Lin, Qiong and Pallua,
Norbert and Zenke, Martin and Merkel, Rudolf and Hoffmann,
Bernd and Wagner, Wolfgang},
title = {{M}atrix elasticity, replicative senescence and {DNA}
methylation patterns of mesenchymal stem cells},
journal = {Biomaterials},
volume = {35},
number = {24},
issn = {0142-9612},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {FZJ-2015-01259},
pages = {6351-6358},
year = {2014},
abstract = {Matrix elasticity guides differentiation of mesenchymal
stem cells (MSCs) but it is unclear if these effects are
only transient – while the cells reside on the substrate
– or if they reflect persistent lineage commitment. In
this study, MSCs were continuously culture-expanded in
parallel either on tissue culture plastic (TCP) or on
polydimethylsiloxane (PDMS) gels of different elasticity to
compare impact on replicative senescence, in vitro
differentiation, gene expression, and DNA methylation (DNAm)
profiles. The maximal number of cumulative population
doublings was not affected by matrix elasticity.
Differentiation towards adipogenic and osteogenic lineage
was increased on soft and rigid biomaterials, respectively
– but this propensity was no more evident if cells were
transferred to TCP. Global gene expression profiles and DNAm
profiles revealed relatively few differences in MSCs
cultured on soft or rigid matrices. Furthermore, only
moderate DNAm changes were observed upon culture on very
soft hydrogels of human platelet lysate. Our results support
the notion that matrix elasticity influences cellular
behavior while the cells reside on the substrate, but it
does not have major impact on cell-intrinsic lineage
determination, replicative senescence or DNAm patterns.},
cin = {ICS-8 / ICS-7},
ddc = {570},
cid = {I:(DE-Juel1)ICS-8-20110106 / I:(DE-Juel1)ICS-7-20110106},
pnm = {453 - Physics of the Cell (POF2-453)},
pid = {G:(DE-HGF)POF2-453},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000338804500020},
pubmed = {pmid:24824582},
doi = {10.1016/j.biomaterials.2014.04.079},
url = {https://juser.fz-juelich.de/record/187633},
}