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000188896 041__ $$aEnglish
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000188896 1001_ $$0P:(DE-HGF)0$$aKempe, Daryan$$b0
000188896 245__ $$aAccurate Fluorescence Quantum Yield Determination by Fluorescence Correlation Spectroscopy
000188896 260__ $$aWashington, DC$$bSoc.$$c2015
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000188896 520__ $$aHere, we present a comparative method for the accurate determination of fluorescence quantum yields (QYs) by fluorescence correlation spectroscopy. By exploiting the high sensitivity of single-molecule spectroscopy, we obtain the QYs of samples in the microliter range and at (sub)nanomolar concentrations. Additionally, in combination with fluorescence lifetime measurements, our method allows the quantification of both static and collisional quenching constants. Thus, besides being simple and fast, our method opens up the possibility to photophysically characterize labeled biomolecules under application-relevant conditions and with low sample consumption, which is often important in single-molecule studies.
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000188896 7001_ $$0P:(DE-Juel1)156122$$aSchöne, Antonie$$b1
000188896 7001_ $$0P:(DE-Juel1)131961$$aFitter, Jörg$$b2$$eCorresponding Author
000188896 7001_ $$0P:(DE-Juel1)140208$$aGabba, Matteo$$b3$$eCorresponding Author
000188896 773__ $$0PERI:(DE-600)2006039-7$$a10.1021/acs.jpcb.5b02170$$gp. 150324100733000 -$$n13$$p4668–4672$$tThe @journal of physical chemistry <Washington, DC> / B$$v119$$x1520-5207$$y2015
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