Journal Article

FZJ-2015-02194

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Accurate Fluorescence Quantum Yield Determination by Fluorescence Correlation Spectroscopy

Kempe, D. ; Schöne, A.FZJ* ; Fitter, J. (Corresponding Author)FZJ* ; Gabba, M. (Corresponding Author)FZJ*

2015
Soc. Washington, DC

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Please use a persistent id in citations: doi:10.1021/acs.jpcb.5b02170

Abstract: Here, we present a comparative method for the accurate determination of fluorescence quantum yields (QYs) by fluorescence correlation spectroscopy. By exploiting the high sensitivity of single-molecule spectroscopy, we obtain the QYs of samples in the microliter range and at (sub)nanomolar concentrations. Additionally, in combination with fluorescence lifetime measurements, our method allows the quantification of both static and collisional quenching constants. Thus, besides being simple and fast, our method opens up the possibility to photophysically characterize labeled biomolecules under application-relevant conditions and with low sample consumption, which is often important in single-molecule studies.

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Contributing Institute(s):

1. Molekulare Biophysik (ICS-5)

Research Program(s):

1. 551 - Functional Macromolecules and Complexes (POF3-551) (POF3-551)

Appears in the scientific report 2015

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Database coverage:
; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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