TY  - JOUR
AU  - Kempe, Daryan
AU  - Schöne, Antonie
AU  - Fitter, Jörg
AU  - Gabba, Matteo
TI  - Accurate Fluorescence Quantum Yield Determination by Fluorescence Correlation Spectroscopy
JO  - The journal of physical chemistry  / B
VL  - 119
IS  - 13
SN  - 1520-5207
CY  - Washington, DC
PB  - Soc.
M1  - FZJ-2015-02194
SP  - 4668–4672
PY  - 2015
AB  - Here, we present a comparative method for the accurate determination of fluorescence quantum yields (QYs) by fluorescence correlation spectroscopy. By exploiting the high sensitivity of single-molecule spectroscopy, we obtain the QYs of samples in the microliter range and at (sub)nanomolar concentrations. Additionally, in combination with fluorescence lifetime measurements, our method allows the quantification of both static and collisional quenching constants. Thus, besides being simple and fast, our method opens up the possibility to photophysically characterize labeled biomolecules under application-relevant conditions and with low sample consumption, which is often important in single-molecule studies.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000352329300013
DO  - DOI:10.1021/acs.jpcb.5b02170
UR  - https://juser.fz-juelich.de/record/188896
ER  -