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@ARTICLE{Kempe:188896,
      author       = {Kempe, Daryan and Schöne, Antonie and Fitter, Jörg and
                      Gabba, Matteo},
      title        = {{A}ccurate {F}luorescence {Q}uantum {Y}ield {D}etermination
                      by {F}luorescence {C}orrelation {S}pectroscopy},
      journal      = {The journal of physical chemistry / B},
      volume       = {119},
      number       = {13},
      issn         = {1520-5207},
      address      = {Washington, DC},
      publisher    = {Soc.},
      reportid     = {FZJ-2015-02194},
      pages        = {4668–4672},
      year         = {2015},
      abstract     = {Here, we present a comparative method for the accurate
                      determination of fluorescence quantum yields (QYs) by
                      fluorescence correlation spectroscopy. By exploiting the
                      high sensitivity of single-molecule spectroscopy, we obtain
                      the QYs of samples in the microliter range and at
                      (sub)nanomolar concentrations. Additionally, in combination
                      with fluorescence lifetime measurements, our method allows
                      the quantification of both static and collisional quenching
                      constants. Thus, besides being simple and fast, our method
                      opens up the possibility to photophysically characterize
                      labeled biomolecules under application-relevant conditions
                      and with low sample consumption, which is often important in
                      single-molecule studies.},
      cin          = {ICS-5},
      ddc          = {530},
      cid          = {I:(DE-Juel1)ICS-5-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000352329300013},
      doi          = {10.1021/acs.jpcb.5b02170},
      url          = {https://juser.fz-juelich.de/record/188896},
}