guest ::
| Home > Publications database > Accurate Fluorescence Quantum Yield Determination by Fluorescence Correlation Spectroscopy > print |
| Home > Publications database > Accurate Fluorescence Quantum Yield Determination by Fluorescence Correlation Spectroscopy > print |
| 001 | 188896 | ||
| 005 | 20240610121154.0 | ||
| 024 | 7 | _ | |a 10.1021/acs.jpcb.5b02170 |2 doi |
| 024 | 7 | _ | |a 1089-5647 |2 ISSN |
| 024 | 7 | _ | |a 1520-5207 |2 ISSN |
| 024 | 7 | _ | |a 1520-6106 |2 ISSN |
| 024 | 7 | _ | |a WOS:000352329300013 |2 WOS |
| 037 | _ | _ | |a FZJ-2015-02194 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 530 |
| 100 | 1 | _ | |a Kempe, Daryan |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Accurate Fluorescence Quantum Yield Determination by Fluorescence Correlation Spectroscopy |
| 260 | _ | _ | |a Washington, DC |c 2015 |b Soc. |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1428592426_24254 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 520 | _ | _ | |a Here, we present a comparative method for the accurate determination of fluorescence quantum yields (QYs) by fluorescence correlation spectroscopy. By exploiting the high sensitivity of single-molecule spectroscopy, we obtain the QYs of samples in the microliter range and at (sub)nanomolar concentrations. Additionally, in combination with fluorescence lifetime measurements, our method allows the quantification of both static and collisional quenching constants. Thus, besides being simple and fast, our method opens up the possibility to photophysically characterize labeled biomolecules under application-relevant conditions and with low sample consumption, which is often important in single-molecule studies. |
| 536 | _ | _ | |a 551 - Functional Macromolecules and Complexes (POF3-551) |0 G:(DE-HGF)POF3-551 |c POF3-551 |x 0 |f POF III |
| 588 | _ | _ | |a Dataset connected to CrossRef, juser.fz-juelich.de |
| 700 | 1 | _ | |a Schöne, Antonie |0 P:(DE-Juel1)156122 |b 1 |
| 700 | 1 | _ | |a Fitter, Jörg |0 P:(DE-Juel1)131961 |b 2 |e Corresponding Author |
| 700 | 1 | _ | |a Gabba, Matteo |0 P:(DE-Juel1)140208 |b 3 |e Corresponding Author |
| 773 | _ | _ | |a 10.1021/acs.jpcb.5b02170 |g p. 150324100733000 - |0 PERI:(DE-600)2006039-7 |n 13 |p 4668–4672 |t The @journal of physical chemistry |v 119 |y 2015 |x 1520-5207 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/188896/files/acs.jpcb.5b02170.pdf |y Restricted |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/188896/files/acs.jpcb.5b02170.pdf?subformat=pdfa |x pdfa |y Restricted |
| 909 | C | O | |o oai:juser.fz-juelich.de:188896 |p VDB |
| 910 | 1 | _ | |a Forschungszentrum Jülich GmbH |0 I:(DE-588b)5008462-8 |k FZJ |b 1 |6 P:(DE-Juel1)156122 |
| 910 | 1 | _ | |a Forschungszentrum Jülich GmbH |0 I:(DE-588b)5008462-8 |k FZJ |b 2 |6 P:(DE-Juel1)131961 |
| 910 | 1 | _ | |a Forschungszentrum Jülich GmbH |0 I:(DE-588b)5008462-8 |k FZJ |b 3 |6 P:(DE-Juel1)140208 |
| 913 | 0 | _ | |a DE-HGF |b Schlüsseltechnologien |l BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung |1 G:(DE-HGF)POF2-450 |0 G:(DE-HGF)POF2-452 |2 G:(DE-HGF)POF2-400 |v Structural Biology |x 0 |
| 913 | 1 | _ | |a DE-HGF |b Key Technologies |l BioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences |1 G:(DE-HGF)POF3-550 |0 G:(DE-HGF)POF3-551 |2 G:(DE-HGF)POF3-500 |v Functional Macromolecules and Complexes |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |
| 914 | 1 | _ | |y 2015 |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0110 |2 StatID |b Science Citation Index |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Thomson Reuters Master Journal List |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0310 |2 StatID |b NCBI Molecular Biology Database |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1150 |2 StatID |b Current Contents - Physical, Chemical and Earth Sciences |
| 915 | _ | _ | |a IF < 5 |0 StatID:(DE-HGF)9900 |2 StatID |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)ICS-5-20110106 |k ICS-5 |l Molekulare Biophysik |x 0 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-5-20110106 |
| 980 | _ | _ | |a UNRESTRICTED |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-6-20200312 |
| 981 | _ | _ | |a I:(DE-Juel1)ER-C-3-20170113 |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|