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| Home > Publications database > A novel isolated Terrabacter-like bacterium can mineralize2-aminopyrimidine, the principal metabolite of microbialsulfadiazine degradation |
| Home > Publications database > A novel isolated Terrabacter-like bacterium can mineralize2-aminopyrimidine, the principal metabolite of microbialsulfadiazine degradation |
| Journal Article | FZJ-2015-02312 |
; ; ; ; ;
2015
Springer Science + Business Media B.V
Dordrecht
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Please use a persistent id in citations: doi:10.1007/s10532-015-9722-9
Abstract: Recently we showed that during thedegradation of sulfadiazine (SDZ) by Microbacteriumlacus strain SDZm4 the principal metabolite2-aminopyrimidine (2-AP) accumulated to the samemolar amount in the culture as SDZ disappeared(Tappe et al. Appl Environ Microbiol 79:2572–2577,2013). Although 2-AP is considered a recalcitrantagent, long-term lysimeter experiments with 14Cpyrimidinelabeled SDZ ([14C]pyrSDZ) provided indicationsfor substantial degradation of the pyrimidinemoiety of the SDZ molecule. Therefore, we aimed toenrich 2-AP degrading bacteria and isolated a pureculture of a Terrabacter-like bacterium, denoted strain2APm3. When provided with 14C-labeled SDZ, M.lacus strain SDZm4 degraded [14C]pyrSDZ to [14C]2-AP. Resting cells of 2APm3 at a concentration of5 9 106 cells ml-1 degraded 62 lM [14C]2-AP tobelow the detection limit (0.6 lM) within 5 days.Disappearance of 2-AP resulted in the production of atleast two transformation products (M1 and M2) withM2 being identified as 2-amino-4-hydroxypyrimidine.After 36 days, the transformation products disappearedand 83 % of the applied [14C]2-AP radioactivitywas trapped as 14CO2. From this we concludethat a consortium of two species should be able toalmost completely degrade SDZ in soils.
Keyword(s): Biology (2nd)
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