%0 Journal Article
%A Claus, Helene L.
%A Walberer, Maureen
%A Simard, Marie L.
%A Emig, Beata
%A Muesken, Sophia M.
%A Rueger, Maria A.
%A Fink, Gereon R.
%A Schroeter, Michael
%T NG2 and NG2-positive cells delineate focal cerebral infarct demarcation in rats
%J Neuropathology
%V 33
%N 1
%@ 0919-6544
%C Oxford [u.a.]
%I Wiley-Blackwell
%M FZJ-2015-03671
%P 30 - 38
%D 2013
%X Focal cerebral ischemia induces cellular responses that may result in secondary tissue damage. We recently demonstrated multi-facetted spatial and temporal patterns of neuroinflammation by multimodal imaging. In the present study, we especially focus on the separation of vital and necrotic tissue, which enabled us to define a demarcation zone. Focal cerebral ischemia was induced via macrosphere embolization of the middle cerebral artery in Wistar rats. Subsequent cellular processes were investigated immunohistochemically from 3 to 56 days after onset of ischemia. We detected several infarct subareas: a necrotic infarct core and its margin adjacent to a nerve/glial antigen 2 (NG2)+ zone delineating it from a vital peri-infarct zone. Initially transition from necrotic to vital tissue was gradual; later on necrosis was precisely separated from vital tissue by a narrow NG2+ belt that was devoid of astrocytes, oligodendrocytes or neurons. Within this demarcation zone NG2+ cells associate with ionized calcium binding adaptor molecule 1 (Iba1) but not with GFAP, neuronal nuclear antigen (NeuN) or 2′, 3′-cyclic nucleotide 3′-phosphodiesterase (CNPase). During further infarct maturation NG2 seemed to be positioned in the extracellular matrix (ECM) of the demarcation zone, whereas Iba1+ cells invaded the necrotic infarct core and GFAP+ cells built a gliotic containing belt between the lesion and NeuN+ unaffected tissue. Overall, our data suggested that NG2 proteoglycan expression and secretion hallmarked demarcation as a process that actively separated necrosis from vital tissue and therefore decisively impacts secondary neurodegeneration after ischemic stroke.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000314472000004
%R 10.1111/j.1440-1789.2012.01322.x
%U https://juser.fz-juelich.de/record/201376