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000201376 1001_ $$0P:(DE-HGF)0$$aClaus, Helene L.$$b0
000201376 245__ $$aNG2 and NG2-positive cells delineate focal cerebral infarct demarcation in rats
000201376 260__ $$aOxford [u.a.]$$bWiley-Blackwell$$c2013
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000201376 520__ $$aFocal cerebral ischemia induces cellular responses that may result in secondary tissue damage. We recently demonstrated multi-facetted spatial and temporal patterns of neuroinflammation by multimodal imaging. In the present study, we especially focus on the separation of vital and necrotic tissue, which enabled us to define a demarcation zone. Focal cerebral ischemia was induced via macrosphere embolization of the middle cerebral artery in Wistar rats. Subsequent cellular processes were investigated immunohistochemically from 3 to 56 days after onset of ischemia. We detected several infarct subareas: a necrotic infarct core and its margin adjacent to a nerve/glial antigen 2 (NG2)+ zone delineating it from a vital peri-infarct zone. Initially transition from necrotic to vital tissue was gradual; later on necrosis was precisely separated from vital tissue by a narrow NG2+ belt that was devoid of astrocytes, oligodendrocytes or neurons. Within this demarcation zone NG2+ cells associate with ionized calcium binding adaptor molecule 1 (Iba1) but not with GFAP, neuronal nuclear antigen (NeuN) or 2′, 3′-cyclic nucleotide 3′-phosphodiesterase (CNPase). During further infarct maturation NG2 seemed to be positioned in the extracellular matrix (ECM) of the demarcation zone, whereas Iba1+ cells invaded the necrotic infarct core and GFAP+ cells built a gliotic containing belt between the lesion and NeuN+ unaffected tissue. Overall, our data suggested that NG2 proteoglycan expression and secretion hallmarked demarcation as a process that actively separated necrosis from vital tissue and therefore decisively impacts secondary neurodegeneration after ischemic stroke.
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000201376 7001_ $$0P:(DE-HGF)0$$aWalberer, Maureen$$b1
000201376 7001_ $$0P:(DE-HGF)0$$aSimard, Marie L.$$b2
000201376 7001_ $$0P:(DE-HGF)0$$aEmig, Beata$$b3
000201376 7001_ $$0P:(DE-HGF)0$$aMuesken, Sophia M.$$b4
000201376 7001_ $$0P:(DE-HGF)0$$aRueger, Maria A.$$b5
000201376 7001_ $$0P:(DE-Juel1)131720$$aFink, Gereon R.$$b6
000201376 7001_ $$0P:(DE-HGF)0$$aSchroeter, Michael$$b7$$eCorresponding Author
000201376 773__ $$0PERI:(DE-600)2008290-3$$a10.1111/j.1440-1789.2012.01322.x$$gVol. 33, no. 1, p. 30 - 38$$n1$$p30 - 38$$tNeuropathology$$v33$$x0919-6544$$y2013
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