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@ARTICLE{Wolf:201767,
      author       = {Wolf, Sharon Grayer and Houben, Lothar and Elbaum, Michael},
      title        = {{C}ryo-scanning transmission electron tomography of
                      vitrified cells},
      journal      = {Nature methods},
      volume       = {11},
      number       = {4},
      issn         = {1548-7105},
      address      = {London [u.a.] Nature Publishing Group},
      publisher    = {Nature Publishing Group},
      reportid     = {FZJ-2015-04061},
      pages        = {423 - 428},
      year         = {2014},
      abstract     = {Cryo-electron tomography (CET) of fully hydrated, vitrified
                      biological specimens has emerged as a vital tool for
                      biological research. For cellular studies, the conventional
                      imaging modality of transmission electron microscopy places
                      stringent constraints on sample thickness because of its
                      dependence on phase coherence for contrast generation. Here
                      we demonstrate the feasibility of using scanning
                      transmission electron microscopy for cryo-tomography of
                      unstained vitrified specimens (CSTET). We compare CSTET and
                      CET for the imaging of whole bacteria and human tissue
                      culture cells, finding favorable contrast and detail in the
                      CSTET reconstructions. Particularly at high sample tilts,
                      the CSTET signals contain more informative data than
                      energy-filtered CET phase contrast images, resulting in
                      improved depth resolution. Careful control over dose
                      delivery permits relatively high cumulative exposures before
                      the onset of observable beam damage. The increase in
                      acceptable specimen thickness broadens the applicability of
                      electron cryo-tomography.},
      cin          = {PGI-5},
      ddc          = {570},
      cid          = {I:(DE-Juel1)PGI-5-20110106},
      pnm          = {42G - Peter Grünberg-Centre (PG-C) (POF2-42G41)},
      pid          = {G:(DE-HGF)POF2-42G41},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000333749900025},
      pubmed       = {pmid:24531421},
      doi          = {10.1038/nmeth.2842},
      url          = {https://juser.fz-juelich.de/record/201767},
}