%0 Journal Article
%A Chen, La
%A Maybeck, Vanessa
%A Offenhäusser, Andreas
%A Krause, Hans-Joachim
%T Characterization of the mechanical properties of HL-1 cardiomyocytes with high throughput magnetic tweezers
%J Applied physics letters
%V 107
%N 5
%@ 1077-3118
%C Melville, NY
%I American Inst. of Physics
%M FZJ-2015-05215
%P 053703 -
%D 2015
%X We characterized the mechanical properties of cardiomyocyte-like HL-1 cells using our recently developed multi-pole magnetic tweezers. With the optimized design, both high force and high throughput are achieved at the same time. Force up to 100 pN can be applied on a 1 μm diameter superparamagnetic bead in a workspace with 60 μm radius, which is encircled symmetrically by 3 sharp magnetic tips. By adjusting the coil currents, both the strength and direction of force can be controlled. The result shows that both viscosity and shear elastic modulus of HL-1 cells exhibit an approximately log-normal distribution. The cells became stiffer as they matured, consistent with a transition from proliferating cells to contractile muscle tissue. Moreover, the mechanical properties of HL-1 cells show high heterogeneity, which agrees well with their physiological structure.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000359375700048
%R 10.1063/1.4928322
%U https://juser.fz-juelich.de/record/203229