TY  - JOUR
AU  - Chen, La
AU  - Maybeck, Vanessa
AU  - Offenhäusser, Andreas
AU  - Krause, Hans-Joachim
TI  - Characterization of the mechanical properties of HL-1 cardiomyocytes with high throughput magnetic tweezers
JO  - Applied physics letters
VL  - 107
IS  - 5
SN  - 1077-3118
CY  - Melville, NY
PB  - American Inst. of Physics
M1  - FZJ-2015-05215
SP  - 053703 -
PY  - 2015
AB  - We characterized the mechanical properties of cardiomyocyte-like HL-1 cells using our recently developed multi-pole magnetic tweezers. With the optimized design, both high force and high throughput are achieved at the same time. Force up to 100 pN can be applied on a 1 μm diameter superparamagnetic bead in a workspace with 60 μm radius, which is encircled symmetrically by 3 sharp magnetic tips. By adjusting the coil currents, both the strength and direction of force can be controlled. The result shows that both viscosity and shear elastic modulus of HL-1 cells exhibit an approximately log-normal distribution. The cells became stiffer as they matured, consistent with a transition from proliferating cells to contractile muscle tissue. Moreover, the mechanical properties of HL-1 cells show high heterogeneity, which agrees well with their physiological structure.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000359375700048
DO  - DOI:10.1063/1.4928322
UR  - https://juser.fz-juelich.de/record/203229
ER  -