Journal Article

PreJuSER-21002

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Repression of early lateral root initiation events by transient water deficit in barley and maize

Babé, A. ; Lavigne, T. ; Séverin, J.-P. ; Nagel, K. A.FZJ* ; Walter, A.FZJ* ; Chaumont, F. ; Batoko, H. ; Beeckman, T. ; Draye, X.

2012
JSTOR London

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Please use a persistent id in citations: doi:10.1098/rstb.2011.0240

Abstract: The formation of lateral roots (LRs) is a key driver of root system architecture and developmental plasticity. The first stage of LR formation, which leads to the acquisition of founder cell identity in the pericycle, is the primary determinant of root branching patterns. The fact that initiation events occur asynchronously in a very small number of cells inside the parent root has been a major difficulty in the study of the molecular regulation of branching patterns. Inducible systems that trigger synchronous lateral formation at predictable sites have proven extremely valuable in Arabidopsis to decipher the first steps of LR formation. Here, we present a LR repression system for cereals that relies on a transient water-deficit treatment, which blocks LR initiation before the first formative divisions. Using a time-lapse approach, we analysed the dynamics of this repression along growing roots and were able to show that it targets a very narrow developmental window of the initiation process. Interestingly, the repression can be exploited to obtain negative control root samples where LR initiation is absent. This system could be instrumental in the analysis of the molecular basis of drought-responsive as well as intrinsic pathways of LR formation in cereals.

Keyword(s): Biological Transport (MeSH) ; Cell Division (MeSH) ; Droughts (MeSH) ; Hordeum: drug effects (MeSH) ; Hordeum: growth & development (MeSH) ; Hordeum: metabolism (MeSH) ; Indoleacetic Acids: metabolism (MeSH) ; Indoleacetic Acids: pharmacology (MeSH) ; Osmotic Pressure (MeSH) ; Plant Cells: metabolism (MeSH) ; Plant Roots: drug effects (MeSH) ; Plant Roots: growth & development (MeSH) ; Plant Roots: metabolism (MeSH) ; Signal Transduction (MeSH) ; Time Factors (MeSH) ; Water: metabolism (MeSH) ; Zea mays: drug effects (MeSH) ; Zea mays: growth & development (MeSH) ; Zea mays: metabolism (MeSH) ; Indoleacetic Acids ; Water ; J ; lateral root initiation (auto) ; water deficit (auto) ; barley (auto) ; maize (auto)

Note: We thank the Communaute francaise de Belgique (ARC 0510-329 grant and FRIA fellowships to A.B. and T.L.) and the Belgian Science Policy (BARN project) for financial support. We are also grateful to Nathalie Wuyts and anonymous referees for constructive comments on a former version of the manuscript.

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Contributing Institute(s):

1. Pflanzenwissenschaften (IBG-2)

Research Program(s):

1. Terrestrische Umwelt (P24)

Appears in the scientific report 2012

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Database coverage:
; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; JCR ; NCBI Molecular Biology Database ; National-Konsortium ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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