TY  - JOUR
AU  - Babé, A.
AU  - Lavigne, T.
AU  - Séverin, J.-P.
AU  - Nagel, K.A.
AU  - Walter, A.
AU  - Chaumont, F.
AU  - Batoko, H.
AU  - Beeckman, T.
AU  - Draye, X.
TI  - Repression of early lateral root initiation events by transient water deficit in barley and maize
JO  - Philosophical Transactions of the Royal Society B: Biological Sciences
VL  - 367
SN  - 0080-4622
CY  - London
PB  - JSTOR
M1  - PreJuSER-21002
SP  - 1534 - 1541
PY  - 2012
N1  - We thank the Communaute francaise de Belgique (ARC 0510-329 grant and FRIA fellowships to A.B. and T.L.) and the Belgian Science Policy (BARN project) for financial support. We are also grateful to Nathalie Wuyts and anonymous referees for constructive comments on a former version of the manuscript.
AB  - The formation of lateral roots (LRs) is a key driver of root system architecture and developmental plasticity. The first stage of LR formation, which leads to the acquisition of founder cell identity in the pericycle, is the primary determinant of root branching patterns. The fact that initiation events occur asynchronously in a very small number of cells inside the parent root has been a major difficulty in the study of the molecular regulation of branching patterns. Inducible systems that trigger synchronous lateral formation at predictable sites have proven extremely valuable in Arabidopsis to decipher the first steps of LR formation. Here, we present a LR repression system for cereals that relies on a transient water-deficit treatment, which blocks LR initiation before the first formative divisions. Using a time-lapse approach, we analysed the dynamics of this repression along growing roots and were able to show that it targets a very narrow developmental window of the initiation process. Interestingly, the repression can be exploited to obtain negative control root samples where LR initiation is absent. This system could be instrumental in the analysis of the molecular basis of drought-responsive as well as intrinsic pathways of LR formation in cereals.
KW  - Biological Transport
KW  - Cell Division
KW  - Droughts
KW  - Hordeum: drug effects
KW  - Hordeum: growth & development
KW  - Hordeum: metabolism
KW  - Indoleacetic Acids: metabolism
KW  - Indoleacetic Acids: pharmacology
KW  - Osmotic Pressure
KW  - Plant Cells: metabolism
KW  - Plant Roots: drug effects
KW  - Plant Roots: growth & development
KW  - Plant Roots: metabolism
KW  - Signal Transduction
KW  - Time Factors
KW  - Water: metabolism
KW  - Zea mays: drug effects
KW  - Zea mays: growth & development
KW  - Zea mays: metabolism
KW  - Indoleacetic Acids (NLM Chemicals)
KW  - Water (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:22527396
C2  - pmc:PMC3321690
UR  - <Go to ISI:>//WOS:000303107900011
DO  - DOI:10.1098/rstb.2011.0240
UR  - https://juser.fz-juelich.de/record/21002
ER  -