%0 Journal Article
%A Yakushenko, A.
%A Schnitker, J.
%A Wolfrum, B.
%T Printed Carbon Microelectrodes for Electrochemical Detection of Single Vesicle Release from PC12 Cells
%J Analytical chemistry
%V 84
%@ 0003-2700
%C Columbus, Ohio
%I American Chemical Society
%M PreJuSER-21511
%P 4613 - 4617
%D 2012
%Z Record converted from VDB: 12.11.2012
%X We present a disposable system for recording neurotransmitter release from individual cells in vitro. A simple yet reliable microelectrode fabrication process is introduced using screen-printed carbon paste. It allows rapid fabrication of devices at low costs without standard clean-room technology. We demonstrate functionality of the system by real-time observation of vesicle release from single PC12 (rat pheochromocytoma) cells. The cells are cultured directly on the chip and can be used for immediate or long-term in vitro experiments. Thus, our approach may serve as a platform for pharmacological cell culture studies.
%K Animals
%K Carbon: chemistry
%K Dopamine: analysis
%K Electrochemical Techniques
%K Microelectrodes
%K Microscopy, Electron, Scanning
%K PC12 Cells
%K Patch-Clamp Techniques
%K Rats
%K Secretory Vesicles: metabolism
%K Carbon (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:22509770
%U <Go to ISI:>//WOS:000303965500052
%R 10.1021/ac300460s
%U https://juser.fz-juelich.de/record/21511