Journal Article

PreJuSER-21511

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Printed Carbon Microelectrodes for Electrochemical Detection of Single Vesicle Release from PC12 Cells

Yakushenko, A.FZJ* ; Schnitker, J.FZJ* ; Wolfrum, B.FZJ*

2012
American Chemical Society Columbus, Ohio

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Please use a persistent id in citations: doi:10.1021/ac300460s

Abstract: We present a disposable system for recording neurotransmitter release from individual cells in vitro. A simple yet reliable microelectrode fabrication process is introduced using screen-printed carbon paste. It allows rapid fabrication of devices at low costs without standard clean-room technology. We demonstrate functionality of the system by real-time observation of vesicle release from single PC12 (rat pheochromocytoma) cells. The cells are cultured directly on the chip and can be used for immediate or long-term in vitro experiments. Thus, our approach may serve as a platform for pharmacological cell culture studies.

Keyword(s): Animals (MeSH) ; Carbon: chemistry (MeSH) ; Dopamine: analysis (MeSH) ; Electrochemical Techniques (MeSH) ; Microelectrodes (MeSH) ; Microscopy, Electron, Scanning (MeSH) ; PC12 Cells (MeSH) ; Patch-Clamp Techniques (MeSH) ; Rats (MeSH) ; Secretory Vesicles: metabolism (MeSH) ; Carbon

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Bioelektronik (ICS-8)
2. Bioelektronik (PGI-8)
3. Jülich-Aachen Research Alliance - Fundamentals of Future Information Technology (JARA-FIT)

Research Program(s):

1. Grundlagen für zukünftige Informationstechnologien (P42)
2. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)

Appears in the scientific report 2012

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Database coverage:
; Current Contents - Life Sciences ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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