Home > Publications database > Printed Carbon Microelectrodes for Electrochemical Detection of Single Vesicle Release from PC12 Cells |
Journal Article | PreJuSER-21511 |
; ;
2012
American Chemical Society
Columbus, Ohio
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Please use a persistent id in citations: doi:10.1021/ac300460s
Abstract: We present a disposable system for recording neurotransmitter release from individual cells in vitro. A simple yet reliable microelectrode fabrication process is introduced using screen-printed carbon paste. It allows rapid fabrication of devices at low costs without standard clean-room technology. We demonstrate functionality of the system by real-time observation of vesicle release from single PC12 (rat pheochromocytoma) cells. The cells are cultured directly on the chip and can be used for immediate or long-term in vitro experiments. Thus, our approach may serve as a platform for pharmacological cell culture studies.
Keyword(s): Animals (MeSH) ; Carbon: chemistry (MeSH) ; Dopamine: analysis (MeSH) ; Electrochemical Techniques (MeSH) ; Microelectrodes (MeSH) ; Microscopy, Electron, Scanning (MeSH) ; PC12 Cells (MeSH) ; Patch-Clamp Techniques (MeSH) ; Rats (MeSH) ; Secretory Vesicles: metabolism (MeSH) ; Carbon
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