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000021511 245__ $$aPrinted Carbon Microelectrodes for Electrochemical Detection of Single Vesicle Release from PC12 Cells
000021511 260__ $$aColumbus, Ohio$$bAmerican Chemical Society$$c2012
000021511 300__ $$a4613 - 4617
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000021511 440_0 $$0364$$aAnalytical Chemistry$$v84$$x0003-2700
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000021511 520__ $$aWe present a disposable system for recording neurotransmitter release from individual cells in vitro. A simple yet reliable microelectrode fabrication process is introduced using screen-printed carbon paste. It allows rapid fabrication of devices at low costs without standard clean-room technology. We demonstrate functionality of the system by real-time observation of vesicle release from single PC12 (rat pheochromocytoma) cells. The cells are cultured directly on the chip and can be used for immediate or long-term in vitro experiments. Thus, our approach may serve as a platform for pharmacological cell culture studies.
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000021511 650_2 $$2MeSH$$aAnimals
000021511 650_2 $$2MeSH$$aCarbon: chemistry
000021511 650_2 $$2MeSH$$aDopamine: analysis
000021511 650_2 $$2MeSH$$aElectrochemical Techniques
000021511 650_2 $$2MeSH$$aMicroelectrodes
000021511 650_2 $$2MeSH$$aMicroscopy, Electron, Scanning
000021511 650_2 $$2MeSH$$aPC12 Cells
000021511 650_2 $$2MeSH$$aPatch-Clamp Techniques
000021511 650_2 $$2MeSH$$aRats
000021511 650_2 $$2MeSH$$aSecretory Vesicles: metabolism
000021511 650_7 $$07440-44-0$$2NLM Chemicals$$aCarbon
000021511 7001_ $$0P:(DE-Juel1)VDB107066$$aSchnitker, J.$$b1$$uFZJ
000021511 7001_ $$0P:(DE-Juel1)128745$$aWolfrum, B.$$b2$$uFZJ
000021511 773__ $$0PERI:(DE-600)1483443-1$$a10.1021/ac300460s$$gVol. 84, p. 4613 - 4617$$p4613 - 4617$$q84<4613 - 4617$$tAnalytical chemistry$$v84$$x0003-2700$$y2012
000021511 8567_ $$uhttp://dx.doi.org/sx.doi.org/10.1021/ac300460s
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