TY  - JOUR
AU  - Yakushenko, A.
AU  - Schnitker, J.
AU  - Wolfrum, B.
TI  - Printed Carbon Microelectrodes for Electrochemical Detection of Single Vesicle Release from PC12 Cells
JO  - Analytical chemistry
VL  - 84
SN  - 0003-2700
CY  - Columbus, Ohio
PB  - American Chemical Society
M1  - PreJuSER-21511
SP  - 4613 - 4617
PY  - 2012
N1  - Record converted from VDB: 12.11.2012
AB  - We present a disposable system for recording neurotransmitter release from individual cells in vitro. A simple yet reliable microelectrode fabrication process is introduced using screen-printed carbon paste. It allows rapid fabrication of devices at low costs without standard clean-room technology. We demonstrate functionality of the system by real-time observation of vesicle release from single PC12 (rat pheochromocytoma) cells. The cells are cultured directly on the chip and can be used for immediate or long-term in vitro experiments. Thus, our approach may serve as a platform for pharmacological cell culture studies.
KW  - Animals
KW  - Carbon: chemistry
KW  - Dopamine: analysis
KW  - Electrochemical Techniques
KW  - Microelectrodes
KW  - Microscopy, Electron, Scanning
KW  - PC12 Cells
KW  - Patch-Clamp Techniques
KW  - Rats
KW  - Secretory Vesicles: metabolism
KW  - Carbon (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:22509770
UR  - <Go to ISI:>//WOS:000303965500052
DO  - DOI:10.1021/ac300460s
UR  - https://juser.fz-juelich.de/record/21511
ER  -