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@ARTICLE{Yakushenko:21511,
author = {Yakushenko, A. and Schnitker, J. and Wolfrum, B.},
title = {{P}rinted {C}arbon {M}icroelectrodes for {E}lectrochemical
{D}etection of {S}ingle {V}esicle {R}elease from {PC}12
{C}ells},
journal = {Analytical chemistry},
volume = {84},
issn = {0003-2700},
address = {Columbus, Ohio},
publisher = {American Chemical Society},
reportid = {PreJuSER-21511},
pages = {4613 - 4617},
year = {2012},
note = {Record converted from VDB: 12.11.2012},
abstract = {We present a disposable system for recording
neurotransmitter release from individual cells in vitro. A
simple yet reliable microelectrode fabrication process is
introduced using screen-printed carbon paste. It allows
rapid fabrication of devices at low costs without standard
clean-room technology. We demonstrate functionality of the
system by real-time observation of vesicle release from
single PC12 (rat pheochromocytoma) cells. The cells are
cultured directly on the chip and can be used for immediate
or long-term in vitro experiments. Thus, our approach may
serve as a platform for pharmacological cell culture
studies.},
keywords = {Animals / Carbon: chemistry / Dopamine: analysis /
Electrochemical Techniques / Microelectrodes / Microscopy,
Electron, Scanning / PC12 Cells / Patch-Clamp Techniques /
Rats / Secretory Vesicles: metabolism / Carbon (NLM
Chemicals)},
cin = {ICS-8 / PGI-8 / JARA-FIT},
ddc = {540},
cid = {I:(DE-Juel1)ICS-8-20110106 / I:(DE-Juel1)PGI-8-20110106 /
$I:(DE-82)080009_20140620$},
pnm = {Grundlagen für zukünftige Informationstechnologien /
BioSoft: Makromolekulare Systeme und biologische
Informationsverarbeitung},
pid = {G:(DE-Juel1)FUEK412 / G:(DE-Juel1)FUEK505},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:22509770},
UT = {WOS:000303965500052},
doi = {10.1021/ac300460s},
url = {https://juser.fz-juelich.de/record/21511},
}