Hauptseite > Publikationsdatenbank > Printed Carbon Microelectrodes for Electrochemical Detection of Single Vesicle Release from PC12 Cells > print

001     21511
005     20240619091013.0
024 7 _ |2 pmid
|a pmid:22509770
024 7 _ |2 DOI
|a 10.1021/ac300460s
024 7 _ |2 WOS
|a WOS:000303965500052
037 _ _ |a PreJuSER-21511
041 _ _ |a eng
082 _ _ |a 540
100 1 _ |0 P:(DE-Juel1)VDB88041
|a Yakushenko, A.
|b 0
|u FZJ
245 _ _ |a Printed Carbon Microelectrodes for Electrochemical Detection of Single Vesicle Release from PC12 Cells
260 _ _ |a Columbus, Ohio
|b American Chemical Society
|c 2012
300 _ _ |a 4613 - 4617
336 7 _ |a Journal Article
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336 7 _ |a article
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440 _ 0 |0 364
|a Analytical Chemistry
|v 84
|x 0003-2700
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a We present a disposable system for recording neurotransmitter release from individual cells in vitro. A simple yet reliable microelectrode fabrication process is introduced using screen-printed carbon paste. It allows rapid fabrication of devices at low costs without standard clean-room technology. We demonstrate functionality of the system by real-time observation of vesicle release from single PC12 (rat pheochromocytoma) cells. The cells are cultured directly on the chip and can be used for immediate or long-term in vitro experiments. Thus, our approach may serve as a platform for pharmacological cell culture studies.
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650 _ 2 |2 MeSH
|a Animals
650 _ 2 |2 MeSH
|a Carbon: chemistry
650 _ 2 |2 MeSH
|a Dopamine: analysis
650 _ 2 |2 MeSH
|a Electrochemical Techniques
650 _ 2 |2 MeSH
|a Microelectrodes
650 _ 2 |2 MeSH
|a Microscopy, Electron, Scanning
650 _ 2 |2 MeSH
|a PC12 Cells
650 _ 2 |2 MeSH
|a Patch-Clamp Techniques
650 _ 2 |2 MeSH
|a Rats
650 _ 2 |2 MeSH
|a Secretory Vesicles: metabolism
650 _ 7 |0 7440-44-0
|2 NLM Chemicals
|a Carbon
700 1 _ |0 P:(DE-Juel1)VDB107066
|a Schnitker, J.
|b 1
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700 1 _ |0 P:(DE-Juel1)128745
|a Wolfrum, B.
|b 2
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773 _ _ |0 PERI:(DE-600)1483443-1
|a 10.1021/ac300460s
|g Vol. 84, p. 4613 - 4617
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|t Analytical chemistry
|v 84
|x 0003-2700
|y 2012
856 7 _ |u http://dx.doi.org/sx.doi.org/10.1021/ac300460s
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