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@ARTICLE{Afanasenkau:22811,
      author       = {Afanasenkau, D. and Offenhäusser, A.},
      title        = {{P}ositively {C}harged {S}upported {L}ipid {B}ilayers as a
                      {B}iomimetric {P}latform for {N}euronal {C}ell {C}ulture},
      journal      = {Langmuir},
      volume       = {28},
      issn         = {0743-7463},
      address      = {Washington, DC},
      publisher    = {ACS Publ.},
      reportid     = {PreJuSER-22811},
      pages        = {13387 - 13394},
      year         = {2012},
      note         = {The authors would like to thank to Dr. Vanessa Maybeck and
                      Alexey Yakushenko for reading and discussing the manuscript,
                      and Ida Delac for the assistance with the experiments. The
                      work was supported by DFG Research Training Group (GRK) 1572
                      Bionik.},
      abstract     = {The supported lipid bilayer (SLB) is a well-known system
                      for studying the cell membrane and membrane proteins. It is
                      also promising as a platform for studying cell processes:
                      the cell adhesion, the cell membrane receptors, and the
                      intercellular signaling processes. SLBs made of natural
                      lipids appeared to be protein and cell repellent. Thus, to
                      use the SLB as a substrate for cells, one should
                      functionalize them to provide adhesion. In the present
                      paper, we describe a simple approach to promote adhesion of
                      neuronal cells to the SLB without using proteins or
                      peptides, by introducing positively charged lipids
                      1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) into the
                      SLB made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
                      (POPC). We show that neurons adhere to these bilayers and
                      grow for at least 10 days. The SLBs themselves were found to
                      degrade with time in cell culture conditions, but maintained
                      fluidity (as revealed by fluorescence recovery after
                      photobleaching), demonstrating the possibility of using SLBs
                      for studying neuronal cells in culture.},
      keywords     = {J (WoSType)},
      cin          = {ICS-8 / PGI-8 / JARA-FIT},
      ddc          = {670},
      cid          = {I:(DE-Juel1)ICS-8-20110106 / I:(DE-Juel1)PGI-8-20110106 /
                      $I:(DE-82)080009_20140620$},
      pnm          = {Grundlagen für zukünftige Informationstechnologien /
                      BioSoft: Makromolekulare Systeme und biologische
                      Informationsverarbeitung},
      pid          = {G:(DE-Juel1)FUEK412 / G:(DE-Juel1)FUEK505},
      shelfmark    = {Chemistry, Multidisciplinary / Chemistry, Physical /
                      Materials Science, Multidisciplinary},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:22920161},
      UT           = {WOS:000308787700032},
      doi          = {10.1021/la302500r},
      url          = {https://juser.fz-juelich.de/record/22811},
}