Journal Article

PreJuSER-22811

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Positively Charged Supported Lipid Bilayers as a Biomimetric Platform for Neuronal Cell Culture

Afanasenkau, D.FZJ* ; Offenhäusser, A.FZJ*

2012
ACS Publ. Washington, DC

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Please use a persistent id in citations: doi:10.1021/la302500r

Abstract: The supported lipid bilayer (SLB) is a well-known system for studying the cell membrane and membrane proteins. It is also promising as a platform for studying cell processes: the cell adhesion, the cell membrane receptors, and the intercellular signaling processes. SLBs made of natural lipids appeared to be protein and cell repellent. Thus, to use the SLB as a substrate for cells, one should functionalize them to provide adhesion. In the present paper, we describe a simple approach to promote adhesion of neuronal cells to the SLB without using proteins or peptides, by introducing positively charged lipids 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) into the SLB made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). We show that neurons adhere to these bilayers and grow for at least 10 days. The SLBs themselves were found to degrade with time in cell culture conditions, but maintained fluidity (as revealed by fluorescence recovery after photobleaching), demonstrating the possibility of using SLBs for studying neuronal cells in culture.

Keyword(s): J

Note: The authors would like to thank to Dr. Vanessa Maybeck and Alexey Yakushenko for reading and discussing the manuscript, and Ida Delac for the assistance with the experiments. The work was supported by DFG Research Training Group (GRK) 1572 Bionik.

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Contributing Institute(s):

1. Bioelektronik (ICS-8)
2. Bioelektronik (PGI-8)
3. Jülich-Aachen Research Alliance - Fundamentals of Future Information Technology (JARA-FIT)

Research Program(s):

1. Grundlagen für zukünftige Informationstechnologien (P42)
2. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)

Appears in the scientific report 2012

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Database coverage:
; Current Contents - Social and Behavioral Sciences ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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