Journal Article

FZJ-2015-06327

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Biosensor-driven adaptive laboratory evolution of l-valine production in $Corynebacterium$ $glutamicum$

Mahr, R.FZJ* ; Gätgens, C. ; Gätgens, J.FZJ* ; Polen, T.FZJ* ; Kalinowski, J. ; Frunzke, J. (Corresponding author)FZJ*

2015
Academic Press Orlando, Fla.

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Please use a persistent id in citations: doi:10.1016/j.ymben.2015.09.017

Abstract: Adaptive laboratory evolution has proven a valuable strategy for metabolic engineering. Here, we established an experimental evolution approach for improving microbial metabolite production by imposing an artificial selective pressure on the fluorescent output of a biosensor using fluorescence-activated cell sorting. Cells showing the highest fluorescent output were iteratively isolated and (re-)cultivated. The l-valine producer Corynebacterium glutamicum ΔaceE was equipped with an L-valine-responsive sensor based on the transcriptional regulator Lrp of C. glutamicum. Evolved strains featured a significantly higher growth rate, increased l-valine titers (~25%) and a 3-4-fold reduction of by-product formation. Genome sequencing resulted in the identification of a loss-of-function mutation (UreD-E188*) in the gene ureD (urease accessory protein), which was shown to increase l-valine production by up to 100%. Furthermore, decreased l-alanine formation was attributed to a mutation in the global regulator GlxR. These results emphasize biosensor-driven evolution as a straightforward approach to improve growth and productivity of microbial production strains.

Note: Biotechnologie 1

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Contributing Institute(s):

1. Biotechnologie (IBG-1)

Research Program(s):

1. 581 - Biotechnology (POF3-581) (POF3-581)

Appears in the scientific report 2015

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Database coverage:
; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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