%0 Journal Article
%A Choe, B. J.
%A Park, K. S.
%A Labahn, J.
%A Granzin, J.
%A Kim, C.-J.
%A Büldt, G.
%T Crystallization and preliminary X-ray diffraction studies of alpha-cyclodextrin glucanotransferase isolated from Bacillus macerans
%J Acta crystallographica / D
%V 59
%@ 0907-4449
%C Copenhagen
%I Munksgaard
%M PreJuSER-26875
%P 348 - 349
%D 2003
%Z Record converted from VDB: 12.11.2012
%X Single crystals of alpha-cyclodextrin glucanotransferase isolated from Bacillus macerans have been grown with polyethylene glycol 6000 as a precipitating agent by sitting-drop vapour diffusion at room temperature. The crystals were suitable for X-ray analysis and diffracted to at least 2.0 A (space group P2(1)2(1)2(1)), with unit-cell parameters a = 66.79 (2), b = 79.66 (1), c = 141.16 (1) A. Assuming the asymmetric cell to be occupied by a monomer of 74 kDa, the unit cell contains 42.6% solvent with a crystal Volume per protein mass, V(M), of 2.53 A(3) Da(-1).
%K Bacillus: enzymology
%K Crystallization: methods
%K Crystallography, X-Ray
%K Glucosyltransferases: chemistry
%K Glucosyltransferases: metabolism
%K Glucosyltransferases (NLM Chemicals)
%K cyclomaltodextrin glucanotransferase (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:12554949
%U <Go to ISI:>//WOS:000180641900019
%R 10.1107/S0907444902020796
%U https://juser.fz-juelich.de/record/26875