Journal Article

PreJuSER-26875

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Crystallization and preliminary X-ray diffraction studies of alpha-cyclodextrin glucanotransferase isolated from Bacillus macerans

Choe, B. J. ; Park, K. S. ; Labahn, J.FZJ* ; Granzin, J.FZJ* ; Kim, C.-J. ; Büldt, G.FZJ*

2003
Munksgaard Copenhagen

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Please use a persistent id in citations: doi:10.1107/S0907444902020796

Abstract: Single crystals of alpha-cyclodextrin glucanotransferase isolated from Bacillus macerans have been grown with polyethylene glycol 6000 as a precipitating agent by sitting-drop vapour diffusion at room temperature. The crystals were suitable for X-ray analysis and diffracted to at least 2.0 A (space group P2(1)2(1)2(1)), with unit-cell parameters a = 66.79 (2), b = 79.66 (1), c = 141.16 (1) A. Assuming the asymmetric cell to be occupied by a monomer of 74 kDa, the unit cell contains 42.6% solvent with a crystal Volume per protein mass, V(M), of 2.53 A(3) Da(-1).

Keyword(s): Bacillus: enzymology (MeSH) ; Crystallization: methods (MeSH) ; Crystallography, X-Ray (MeSH) ; Glucosyltransferases: chemistry (MeSH) ; Glucosyltransferases: metabolism (MeSH) ; Glucosyltransferases ; cyclomaltodextrin glucanotransferase ; J

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Biologische Strukturforschung (IBI-2)

Research Program(s):

1. Neurowissenschaften (L01)

Appears in the scientific report 2003

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