TY  - JOUR
AU  - Choe, B. J.
AU  - Park, K. S.
AU  - Labahn, J.
AU  - Granzin, J.
AU  - Kim, C.-J.
AU  - Büldt, G.
TI  - Crystallization and preliminary X-ray diffraction studies of alpha-cyclodextrin glucanotransferase isolated from Bacillus macerans
JO  - Acta crystallographica / D
VL  - 59
SN  - 0907-4449
CY  - Copenhagen
PB  - Munksgaard
M1  - PreJuSER-26875
SP  - 348 - 349
PY  - 2003
N1  - Record converted from VDB: 12.11.2012
AB  - Single crystals of alpha-cyclodextrin glucanotransferase isolated from Bacillus macerans have been grown with polyethylene glycol 6000 as a precipitating agent by sitting-drop vapour diffusion at room temperature. The crystals were suitable for X-ray analysis and diffracted to at least 2.0 A (space group P2(1)2(1)2(1)), with unit-cell parameters a = 66.79 (2), b = 79.66 (1), c = 141.16 (1) A. Assuming the asymmetric cell to be occupied by a monomer of 74 kDa, the unit cell contains 42.6% solvent with a crystal Volume per protein mass, V(M), of 2.53 A(3) Da(-1).
KW  - Bacillus: enzymology
KW  - Crystallization: methods
KW  - Crystallography, X-Ray
KW  - Glucosyltransferases: chemistry
KW  - Glucosyltransferases: metabolism
KW  - Glucosyltransferases (NLM Chemicals)
KW  - cyclomaltodextrin glucanotransferase (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:12554949
UR  - <Go to ISI:>//WOS:000180641900019
DO  - DOI:10.1107/S0907444902020796
UR  - https://juser.fz-juelich.de/record/26875
ER  -