Journal Article

FZJ-2015-07224

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Nucleation and Growth of Membrane Protein Crystals In Meso —A Fluorescence Microscopy Study

Bogorodskiy, A. ; Frolov, F. ; Mishin, A. ; Round, E. ; Polovinkin, V. ; Cherezov, V. ; Gordeliy, V.FZJ* ; Büldt, G.FZJ* ; Gensch, T.FZJ* ; Borshchevskiy, V. (Corresponding author)

2015
ACS Publ. Washington, DC

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Please use a persistent id in citations: doi:10.1021/acs.cgd.5b01061

Abstract: Since the introduction of in meso crystallization of membrane proteins in lipidic cubic phase (LCP) by Landau and Rosenbusch in 1996, numerous studies attempted to elucidate the mechanism of in meso crystal nucleation and growth. Here we present a fluorescence microscopy study of the crystallization process of the light-driven proton pump bacteriorhodopsin. The crystallization starts with formation of microcrystals, followed by growth of a dominating crystal at the expense of smaller ones and formation of a depletion zone around it. These observations suggest an Ostwald ripening mechanism of the in meso crystal growth. Analysis of the microcrystal spatial distribution suggests that microcrystal nucleation occurs predominately at the LCP domain boundaries.

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Contributing Institute(s):

1. Zelluläre Biophysik (ICS-4)
2. Molekulare Biophysik (ICS-5)
3. Strukturbiochemie (ICS-6)

Research Program(s):

1. 552 - Engineering Cell Function (POF3-552) (POF3-552)

Appears in the scientific report 2015

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Database coverage:
; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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