TY  - JOUR
AU  - Zander, Ulrich
AU  - Bourenkov, Gleb
AU  - Popov, Alexander N.
AU  - de Sanctis, Daniele
AU  - Svensson, Olof
AU  - McCarthy, Andrew A.
AU  - Round, Ekaterina
AU  - Gordeliy, Valentin
AU  - Mueller-Dieckmann, Christoph
AU  - Leonard, Gordon A.
TI  - MeshAndCollect : an automated multi-crystal data-collection workflow for synchrotron macromolecular crystallography beamlines
JO  - Acta crystallographica / D
VL  - 71
IS  - 11
SN  - 1399-0047
CY  - Oxford
PB  - Wiley-Blackwell
M1  - FZJ-2015-07499
SP  - 2328 - 2343
PY  - 2015
AB  - Here, an automated procedure is described to identify the positions of many cryocooled crystals mounted on the same sample holder, to rapidly predict and rank their relative diffraction strengths and to collect partial X-ray diffraction data sets from as many of the crystals as desired. Subsequent hierarchical cluster analysis then allows the best combination of partial data sets, optimizing the quality of the final data set obtained. The results of applying the method developed to various systems and scenarios including the compilation of a complete data set from tiny crystals of the membrane protein bacteriorhodopsin and the collection of data sets for successful structure determination using the single-wavelength anomalous dispersion technique are also presented.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000364553500014
C6  - pmid:26527148
DO  - DOI:10.1107/S1399004715017927
UR  - https://juser.fz-juelich.de/record/279615
ER  -