Journal Article

FZJ-2015-07590

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Cutting the gordian knot: identifiability of anaplerotic reactions in Corynebacterium glutamicum by means of $^{13}$C-metabolic flux analysis

Kappelmann, J.FZJ* ; Wiechert, W.FZJ* ; Noack, S. (Corresponding author)FZJ*

2016
Wiley New York, NY [u.a.]

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Please use a persistent id in citations: doi:10.1002/bit.25833

Abstract: Corynebacterium glutamicum is the major workhorse for the microbial production of several amino and organic acids. As long as these derive from tricarboxylic acid cycle intermediates, the activity of anaplerotic reactions is pivotal for a high biosynthetic yield. To determine single anaplerotic activities 13C-Metabolic Flux Analysis (13C-MFA) has been extensively used for C. glutamicum, however with different network topologies, inconsistent or poorly determined anaplerotic reaction rates. Therefore, in this study we set out to investigate whether a focused isotopomer model of the anaplerotic node can at all admit a unique solution for all fluxes. By analyzing different scenarios of active anaplerotic reactions, we show in full generality that for C. glutamicum only certain anaplerotic deletion mutants allow to uniquely determine the anaplerotic fluxes from 13C-isotopomer data. We stress that the result of this analysis for different assumptions on active enzymes is directly transferable to other compartment-free organisms. Our results demonstrate that there exist biologically relevant metabolic network topologies for which the flux distribution cannot be inferred by classical 13C-MFA

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Contributing Institute(s):

1. Biotechnologie (IBG-1)

Research Program(s):

1. 583 - Innovative Synergisms (POF3-583) (POF3-583)

Appears in the scientific report 2016

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Database coverage:
; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; No Authors Fulltext ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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