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@ARTICLE{Bhler:280979,
author = {Bühler, Jonas and Rishmawi, Louai and Pflugfelder, Daniel
and Huber, Gregor and Scharr, Hanno and Hülskamp, Martin
and Koornneef, Maarten and Schurr, Ulrich and Jahnke,
Siegfried},
title = {pheno{V}ein - {A} tool for leaf vein segmentation and
analysis},
journal = {Plant physiology},
volume = {169},
number = {4},
issn = {1532-2548},
publisher = {American Society of Plant Biologists},
reportid = {FZJ-2016-00690},
pages = {2359–2370},
year = {2015},
abstract = {Precise measurements of leaf vein traits are an important
aspect of plant phenotyping for ecological and genetic
research. Here, we present a powerful and user-friendly
image analysis tool named phenoVein. It is dedicated to
automated segmenting and analyzing of leaf veins in images
acquired with different imaging modalities (microscope,
macrophotography, etc.), including options for comfortable
manual correction. Advanced image filtering emphasizes veins
from the background and compensates for local brightness
inhomogeneities. The most important traits being calculated
are total vein length, vein density, piecewise vein lengths
and widths, areole area, and skeleton graph statistics, like
the number of branching or ending points. For the
determination of vein widths, a model-based vein edge
estimation approach has been implemented. Validation was
performed for the measurement of vein length, vein width,
and vein density of Arabidopsis (Arabidopsis thaliana),
proving the reliability of phenoVein. We demonstrate the
power of phenoVein on a set of previously described vein
structure mutants of Arabidopsis (hemivenata, ondulata3, and
asymmetric leaves2-101) compared with wild-type accessions
Columbia-0 and Landsberg erecta-0. phenoVein is freely
available as open-source software.},
cin = {IBG-2},
ddc = {580},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {582 - Plant Science (POF3-582) / ALCUE-KBBE - Towards a
Latin America $\&$ Caribbean Knowledge Based Bio-Economy
(KBBE) in partnership with Europe (264266) / DPPN -
Deutsches Pflanzen Phänotypisierungsnetzwerk
(BMBF-031A053A)},
pid = {G:(DE-HGF)POF3-582 / G:(EU-Grant)264266 /
G:(DE-Juel1)BMBF-031A053A},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000368472700003},
pubmed = {pmid:26468519},
doi = {10.1104/pp.15.00974},
url = {https://juser.fz-juelich.de/record/280979},
}