Journal Article

FZJ-2016-00690

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png phenoVein - A tool for leaf vein segmentation and analysis

Bühler, J. (Corresponding author)FZJ* ; Rishmawi, L. ; Pflugfelder, D.FZJ* ; Huber, G.FZJ* ; Scharr, H.FZJ* ; Hülskamp, M. ; Koornneef, M. ; Schurr, U.FZJ* ; Jahnke, S.FZJ*

2015
American Society of Plant Biologists

This record in other databases:      

Please use a persistent id in citations: http://hdl.handle.net/2128/9811  doi:10.1104/pp.15.00974

Abstract: Precise measurements of leaf vein traits are an important aspect of plant phenotyping for ecological and genetic research. Here, we present a powerful and user-friendly image analysis tool named phenoVein. It is dedicated to automated segmenting and analyzing of leaf veins in images acquired with different imaging modalities (microscope, macrophotography, etc.), including options for comfortable manual correction. Advanced image filtering emphasizes veins from the background and compensates for local brightness inhomogeneities. The most important traits being calculated are total vein length, vein density, piecewise vein lengths and widths, areole area, and skeleton graph statistics, like the number of branching or ending points. For the determination of vein widths, a model-based vein edge estimation approach has been implemented. Validation was performed for the measurement of vein length, vein width, and vein density of Arabidopsis (Arabidopsis thaliana), proving the reliability of phenoVein. We demonstrate the power of phenoVein on a set of previously described vein structure mutants of Arabidopsis (hemivenata, ondulata3, and asymmetric leaves2-101) compared with wild-type accessions Columbia-0 and Landsberg erecta-0. phenoVein is freely available as open-source software.

---

Contributing Institute(s):

1. Pflanzenwissenschaften (IBG-2)

Research Program(s):

1. 582 - Plant Science (POF3-582) (POF3-582)
2. ALCUE-KBBE - Towards a Latin America & Caribbean Knowledge Based Bio-Economy (KBBE) in partnership with Europe (264266) (264266)
3. DPPN - Deutsches Pflanzen Phänotypisierungsnetzwerk (BMBF-031A053A) (BMBF-031A053A)

Appears in the scientific report 2015

---

Database coverage:
; ; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Free to read ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; National-Konsortium ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

---