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@INPROCEEDINGS{Lenz:283344,
      author       = {Lenz, Henning and Putz, Alexander and Kleinen, Silke and
                      Kastenholz, Bernd and Fiorani, Fabio and Nagel, Kerstin},
      title        = {{D}evelopment of a high-throughput agar-based root and
                      shoot phenotyping system},
      reportid     = {FZJ-2016-01772},
      year         = {2016},
      abstract     = {Roots are most of the time not directly visible, but are of
                      outstanding importance for plant nutrition. As we are using
                      plants for our nutrition and other economic needs there are
                      as well of big importance for everybody. Nevertheless plant
                      research has not been driven very much by roots as this
                      always implies the problem of monitoring, measuring and
                      evaluating a well-hidden belowground structure which has to
                      be made accessible for these tasks. Gathering a deeper
                      knowledge about root structure, development and behavior is
                      important because this will influence our possibilities of
                      improving plant productivity and usability under stress
                      conditions (e.g. limited nutrients, water, salt) in the
                      future.For this reason several screening systems have been
                      developed in recent years which are trying to overcome the
                      problem of visibility and throughput to cope with the need
                      for screening huge plant collections either for getting
                      basic knowledge or for finding root traits useable in plant
                      breeding. One of these systems is an agar based 2D system
                      where seedlings or smaller plants can be grown in
                      transparent media with defined composition while being able
                      to monitor whole root development inside and shoot
                      development outside of the container at the same time. In
                      the present system the plants are grown in commercially
                      available Petri dishes which have to be modified to allow
                      the shoot to grow outside the plate. Individual plate
                      preparation is quite time consuming and therefore not
                      suitable for high throughput phenotyping. Consequently, one
                      goal for the high throughput installation, which is
                      currently developed within the DPPN project, is a reduction
                      of manual production. To solve this we are currently testing
                      special designed agar-filled plates, in parallel, various
                      transport and imaging concepts are evaluated. The current
                      concept is a modular design in which the agar-filled plates
                      will be grouped in trays, in which they will be transported
                      to the imaging station for shoot and root measurements. This
                      concept allows also randomization and will enable screening
                      of thousands of plants without manual interaction.
                      Furthermore, the installation will also be useable for
                      phenotyping plants grown in soil filled pots which makes it
                      flexible for different types of experimental questions.},
      month         = {Mar},
      date          = {2016-03-14},
      organization  = {PLANT 2030 Status Seminar, Potsdam
                       (Germany), 14 Mar 2016 - 16 Mar 2016},
      subtyp        = {After Call},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582) / DPPN - Deutsches Pflanzen
                      Phänotypisierungsnetzwerk (BMBF-031A053A)},
      pid          = {G:(DE-HGF)POF3-582 / G:(DE-Juel1)BMBF-031A053A},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/283344},
}