Journal Article

PreJuSER-341

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Precise measurement of diffusion by multi-color dual-focus fluorescence correlation spectroscopy

Müller, C. B. ; Loman, A. ; Pacheco, V.FZJ* ; Koberling, F. ; Willbold, D.FZJ* ; Richtering, W. ; Enderlein, J.

2008
EDP Sciences Les Ulis

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Please use a persistent id in citations: doi:10.1209/0295-5075/83/46001

Abstract: Dual-focus. uorescence correlation spectroscopy is a method for precisely measuring the diffusion coefficient of fluorescing molecules close to the infinite dilution limit in a reference-free and absolute manner. We apply the method to determine the diffusion coefficients of three fluorescent dyes across the visible spectrum. These values can be used as absolute reference standards for. uorescence correlation spectroscopy. In particular, it is found that the diffusion coefficient of the widely used reference dye Rhodamine 6G is by 37% larger than the value used in most publications on. uorescence correlation spectroscopy over the last three decades. Copyright (c) EPLA, 2008.

Keyword(s): J

Note: Financial support by the Deutsche Forschungsgemeinschaft (SPP1259) and the Deutsche Volkswagenstiftung (JE) is gratefully acknowledged.

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Contributing Institute(s):

1. Molekulare Biophysik (INB-2)
2. Jülich-Aachen Research Alliance - Simulation Sciences (JARA-SIM)

Research Program(s):

1. Funktion und Dysfunktion des Nervensystems (P33)

Appears in the scientific report 2008

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Database coverage:
JCR ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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