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024 7 _ |2 DOI
|a 10.1209/0295-5075/83/46001
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041 _ _ |a eng
082 _ _ |a 530
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|a Physics, Multidisciplinary
100 1 _ |0 P:(DE-HGF)0
|a Müller, C. B.
|b 0
245 _ _ |a Precise measurement of diffusion by multi-color dual-focus fluorescence correlation spectroscopy
260 _ _ |a Les Ulis
|b EDP Sciences
|c 2008
300 _ _ |a 46001
336 7 _ |a Journal Article
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336 7 _ |a article
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440 _ 0 |0 1996
|a Europhysics Letters
|v 83
|x 0295-5075
500 _ _ |a Financial support by the Deutsche Forschungsgemeinschaft (SPP1259) and the Deutsche Volkswagenstiftung (JE) is gratefully acknowledged.
520 _ _ |a Dual-focus. uorescence correlation spectroscopy is a method for precisely measuring the diffusion coefficient of fluorescing molecules close to the infinite dilution limit in a reference-free and absolute manner. We apply the method to determine the diffusion coefficients of three fluorescent dyes across the visible spectrum. These values can be used as absolute reference standards for. uorescence correlation spectroscopy. In particular, it is found that the diffusion coefficient of the widely used reference dye Rhodamine 6G is by 37% larger than the value used in most publications on. uorescence correlation spectroscopy over the last three decades. Copyright (c) EPLA, 2008.
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|a Richtering, W.
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|a Enderlein, J.
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