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| Home > Publications database > Process strategies to enhance pyruvate production with recombinant Escherichia coli: From repetitive fed-batch to in situ product recovery with fully integrated electrodialysis |
| Journal Article | PreJuSER-34886 |
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2004
Wiley
New York, NY [u.a.]
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Please use a persistent id in citations: doi:10.1002/bit.108.20
Abstract: Using the pyruvate production strain Escherichia coli YYC202 IdhA::Kan different process alternatives are studied with the aim of preventing potential product inhibition by appropriate product separation. This strain is completely blocked in its ability to convert pyruvate into acetyl-CoA or acetate, resulting in acetate auxotrophy during growth in glucose minimal medium. Continuous experiments with cell retention, repetitive fed-batch, and an in situ product recovery (ISPR) process with fully integrated electrodialysis were tested. Although the continuous approach achieved a high volumetric productivity (Q(P)) of 110 g L-1 d(-1), this approach was not pursued because of long-term production strain instabilities. The highest pyruvate/glucose molar yield of up to 1.78 mol mol(-1) together with high Q(P) 145 g L-1 d(-1) and high pyruvate titers was achieved by the repetitive fed-batch approach. To separate pyruvate from fermentation broth a fully integrated continuous process was developed. In this process electrodialysis was used as a separation unit. Under optimum conditions a (calculated) final pyruvate titer of >900 mmol L-1 (79 g L-1) was achieved. (C) 2004 Wiley Periodicals, Inc.
Keyword(s): J ; pyruvate (auto) ; E. coli (auto) ; repetitive fed-batch (auto) ; cell retention (auto) ; electrodialysis (auto) ; in situ product recovery (ISPR) (auto)
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