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000034886 0247_ $$2DOI$$a10.1002/bit.108.20
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000034886 084__ $$2WoS$$aBiotechnology & Applied Microbiology
000034886 1001_ $$0P:(DE-HGF)0$$aZelic, B.$$b0
000034886 245__ $$aProcess strategies to enhance pyruvate production with recombinant Escherichia coli: From repetitive fed-batch to in situ product recovery with fully integrated electrodialysis
000034886 260__ $$aNew York, NY [u.a.]$$bWiley$$c2004
000034886 300__ $$a638 - 646
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000034886 440_0 $$0900$$aBiotechnology and Bioengineering$$v85$$x0006-3592$$y6
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000034886 520__ $$aUsing the pyruvate production strain Escherichia coli YYC202 IdhA::Kan different process alternatives are studied with the aim of preventing potential product inhibition by appropriate product separation. This strain is completely blocked in its ability to convert pyruvate into acetyl-CoA or acetate, resulting in acetate auxotrophy during growth in glucose minimal medium. Continuous experiments with cell retention, repetitive fed-batch, and an in situ product recovery (ISPR) process with fully integrated electrodialysis were tested. Although the continuous approach achieved a high volumetric productivity (Q(P)) of 110 g L-1 d(-1), this approach was not pursued because of long-term production strain instabilities. The highest pyruvate/glucose molar yield of up to 1.78 mol mol(-1) together with high Q(P) 145 g L-1 d(-1) and high pyruvate titers was achieved by the repetitive fed-batch approach. To separate pyruvate from fermentation broth a fully integrated continuous process was developed. In this process electrodialysis was used as a separation unit. Under optimum conditions a (calculated) final pyruvate titer of >900 mmol L-1 (79 g L-1) was achieved. (C) 2004 Wiley Periodicals, Inc.
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000034886 65320 $$2Author$$apyruvate
000034886 65320 $$2Author$$aE. coli
000034886 65320 $$2Author$$arepetitive fed-batch
000034886 65320 $$2Author$$acell retention
000034886 65320 $$2Author$$aelectrodialysis
000034886 65320 $$2Author$$ain situ product recovery (ISPR)
000034886 7001_ $$0P:(DE-HGF)0$$aGostovic, S.$$b1
000034886 7001_ $$0P:(DE-HGF)0$$aVuorilehto, K.$$b2
000034886 7001_ $$0P:(DE-HGF)0$$aVasic-Racki, D.$$b3
000034886 7001_ $$0P:(DE-Juel1)VDB1625$$aTakors, R.$$b4$$uFZJ
000034886 773__ $$0PERI:(DE-600)1480809-2$$a10.1002/bit.108.20$$gVol. 85, p. 638 - 646$$p638 - 646$$q85<638 - 646$$tBiotechnology & bioengineering$$v85$$x0006-3592$$y2004
000034886 8567_ $$uhttp://dx.doi.org/10.1002/bit.108.20
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