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@ARTICLE{Zelic:34886,
      author       = {Zelic, B. and Gostovic, S. and Vuorilehto, K. and
                      Vasic-Racki, D. and Takors, R.},
      title        = {{P}rocess strategies to enhance pyruvate production with
                      recombinant {E}scherichia coli: {F}rom repetitive fed-batch
                      to in situ product recovery with fully integrated
                      electrodialysis},
      journal      = {Biotechnology $\&$ bioengineering},
      volume       = {85},
      issn         = {0006-3592},
      address      = {New York, NY [u.a.]},
      publisher    = {Wiley},
      reportid     = {PreJuSER-34886},
      pages        = {638 - 646},
      year         = {2004},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Using the pyruvate production strain Escherichia coli
                      YYC202 IdhA::Kan different process alternatives are studied
                      with the aim of preventing potential product inhibition by
                      appropriate product separation. This strain is completely
                      blocked in its ability to convert pyruvate into acetyl-CoA
                      or acetate, resulting in acetate auxotrophy during growth in
                      glucose minimal medium. Continuous experiments with cell
                      retention, repetitive fed-batch, and an in situ product
                      recovery (ISPR) process with fully integrated
                      electrodialysis were tested. Although the continuous
                      approach achieved a high volumetric productivity (Q(P)) of
                      110 g L-1 d(-1), this approach was not pursued because of
                      long-term production strain instabilities. The highest
                      pyruvate/glucose molar yield of up to 1.78 mol mol(-1)
                      together with high Q(P) 145 g L-1 d(-1) and high pyruvate
                      titers was achieved by the repetitive fed-batch approach. To
                      separate pyruvate from fermentation broth a fully integrated
                      continuous process was developed. In this process
                      electrodialysis was used as a separation unit. Under optimum
                      conditions a (calculated) final pyruvate titer of >900 mmol
                      L-1 (79 g L-1) was achieved. (C) 2004 Wiley Periodicals,
                      Inc.},
      keywords     = {J (WoSType)},
      cin          = {IBT-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB56},
      pnm          = {Biotechnologie},
      pid          = {G:(DE-Juel1)FUEK256},
      shelfmark    = {Biotechnology $\&$ Applied Microbiology},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000189158400008},
      doi          = {10.1002/bit.108.20},
      url          = {https://juser.fz-juelich.de/record/34886},
}