%0 Journal Article
%A Takors, R.
%T Model-based analysis and optimization of an ISPR approach using reactive extraction for pilot scale L-Phenylalanine production
%J Biotechnology progress
%V 20
%@ 8756-7938
%C Malden, MA
%I Wiley
%M PreJuSER-34923
%P 57 - 64
%D 2004
%Z Record converted from VDB: 12.11.2012
%X Based on experimental data from fermentation runs, as well as from L-phenylalanine (l-Phe) separation studies, a simple model is presented that describes the total ISPR approach for on-line L-Phe separation. While fermentation process modeling via a macrokinetic model revealed an L-Phe inhibition constant of 20 +/- 1.35 g/L using recombinant E. coli cells, the reactive-extraction process modeling identified the L-Phe cation diffusion in the aqueous donor film and the transport of the lowly soluble carrier/L-Phe complex in the aqueous acceptor film as the most dominant transfer steps. The corresponding mass transfer coefficients were estimated as k(PheD) = 128 x 10(-7) cm/s (extraction) and k(CPheA) = 178 x 10(-5) cm/s (back-extraction). Simulation studies were performed for the total ISPR approach, which gave hints for strategies of further process optimization.
%K Algorithms
%K Bioreactors: microbiology
%K Computer Simulation
%K Escherichia coli: growth & development
%K Escherichia coli: metabolism
%K Fractional Precipitation
%K Industrial Microbiology: methods
%K Kinetics
%K Models, Biological
%K Models, Chemical
%K Phenylalanine: biosynthesis
%K Phenylalanine: chemistry
%K Phenylalanine: isolation & purification
%K Pilot Projects
%K Quality Control
%K Ultrafiltration: methods
%K Phenylalanine (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:14763824
%U <Go to ISI:>//WOS:000188861300008
%R 10.1021/bp0257473
%U https://juser.fz-juelich.de/record/34923