Model-based analysis and optimization of an ISPR approach using reactive extraction for pilot scale L-Phenylalanine production

Takors, R.

doi:10.1021/bp0257473

Journal Article

Model-based analysis and optimization of an ISPR approach using reactive extraction for pilot scale L-Phenylalanine production

Takors, R.FZJ*

2004
Wiley Malden, MA

Biotechnology progress 20, 57 - 64 (2004) [10.1021/bp0257473]

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Please use a persistent id in citations: http://hdl.handle.net/2128/2440 doi:10.1021/bp0257473

Abstract: Based on experimental data from fermentation runs, as well as from L-phenylalanine (l-Phe) separation studies, a simple model is presented that describes the total ISPR approach for on-line L-Phe separation. While fermentation process modeling via a macrokinetic model revealed an L-Phe inhibition constant of 20 +/- 1.35 g/L using recombinant E. coli cells, the reactive-extraction process modeling identified the L-Phe cation diffusion in the aqueous donor film and the transport of the lowly soluble carrier/L-Phe complex in the aqueous acceptor film as the most dominant transfer steps. The corresponding mass transfer coefficients were estimated as k(PheD) = 128 x 10(-7) cm/s (extraction) and k(CPheA) = 178 x 10(-5) cm/s (back-extraction). Simulation studies were performed for the total ISPR approach, which gave hints for strategies of further process optimization.

Keyword(s): Algorithms (MeSH) ; Bioreactors: microbiology (MeSH) ; Computer Simulation (MeSH) ; Escherichia coli: growth & development (MeSH) ; Escherichia coli: metabolism (MeSH) ; Fractional Precipitation (MeSH) ; Industrial Microbiology: methods (MeSH) ; Kinetics (MeSH) ; Models, Biological (MeSH) ; Models, Chemical (MeSH) ; Phenylalanine: biosynthesis (MeSH) ; Phenylalanine: chemistry (MeSH) ; Phenylalanine: isolation & purification (MeSH) ; Pilot Projects (MeSH) ; Quality Control (MeSH) ; Ultrafiltration: methods (MeSH) ; Phenylalanine ; J