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000038306 084__ $$2WoS$$aBiochemistry & Molecular Biology
000038306 084__ $$2WoS$$aPharmacology & Pharmacy
000038306 1001_ $$0P:(DE-Juel1)VDB8451$$aEnderlein, J.$$b0$$uFZJ
000038306 245__ $$aArt and artefacts of fluorescence correlation spectroscopy
000038306 260__ $$aHilversum$$bBentham Science Publ.$$c2004
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000038306 440_0 $$011902$$aCurrent Pharmaceutical Biotechnology$$v5$$x1389-2010
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000038306 520__ $$aFluorescence correlation spectroscopy (FCS) is an important technique for studying low concentrations of analyte molecules in solution. The core molecular characteristic that can be addressed by FCS is the translational diffusion coefficient or the analyte molecules, which can be used for i.e. studying molecular binding and reactions, or conformational changes of macromolecules. The present paper discusses several possible optical and photophysical effects that can influence the outcome of a FCS measurement and thus can bias the value of the derived diffusion coefficient.
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000038306 65320 $$2Author$$afluorescence correlation spectroscopy
000038306 65320 $$2Author$$adiffusion coefficients
000038306 65320 $$2Author$$aconfocal detection
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000038306 7001_ $$0P:(DE-Juel1)VDB27456$$aGregor, I.$$b1$$uFZJ
000038306 7001_ $$0P:(DE-Juel1)VDB27457$$aPatra, D.$$b2$$uFZJ
000038306 7001_ $$0P:(DE-Juel1)131961$$aFitter, J.$$b3$$uFZJ
000038306 773__ $$0PERI:(DE-600)2044611-1$$a10.2174/1389201043377020$$gVol. 5, p. 155 - 161$$n2$$p155 - 161$$q5<155 - 161$$tCurrent pharmaceutical biotechnology$$v5$$x1389-2010$$y2004
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