Journal Article

PreJuSER-38306

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Art and artefacts of fluorescence correlation spectroscopy

Enderlein, J.FZJ* ; Gregor, I.FZJ* ; Patra, D.FZJ* ; Fitter, J.FZJ*

2004
Bentham Science Publ. Hilversum

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Please use a persistent id in citations: doi:10.2174/1389201043377020

Abstract: Fluorescence correlation spectroscopy (FCS) is an important technique for studying low concentrations of analyte molecules in solution. The core molecular characteristic that can be addressed by FCS is the translational diffusion coefficient or the analyte molecules, which can be used for i.e. studying molecular binding and reactions, or conformational changes of macromolecules. The present paper discusses several possible optical and photophysical effects that can influence the outcome of a FCS measurement and thus can bias the value of the derived diffusion coefficient.

Keyword(s): J ; fluorescence correlation spectroscopy (auto) ; diffusion coefficients (auto) ; confocal detection (auto)

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Zelluläre Signalverarbeitung (IBI-1)
2. Biologische Strukturforschung (IBI-2)

Research Program(s):

1. Neurowissenschaften (L01)

Appears in the scientific report 2004

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