TY  - JOUR
AU  - Enderlein, J.
AU  - Gregor, I.
AU  - Patra, D.
AU  - Fitter, J.
TI  - Art and artefacts of fluorescence correlation spectroscopy
JO  - Current pharmaceutical biotechnology
VL  - 5
IS  - 2
SN  - 1389-2010
CY  - Hilversum
PB  - Bentham Science Publ.
M1  - PreJuSER-38306
SP  - 155 - 161
PY  - 2004
N1  - Record converted from VDB: 12.11.2012
AB  - Fluorescence correlation spectroscopy (FCS) is an important technique for studying low concentrations of analyte molecules in solution. The core molecular characteristic that can be addressed by FCS is the translational diffusion coefficient or the analyte molecules, which can be used for i.e. studying molecular binding and reactions, or conformational changes of macromolecules. The present paper discusses several possible optical and photophysical effects that can influence the outcome of a FCS measurement and thus can bias the value of the derived diffusion coefficient.
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000224302600004
DO  - DOI:10.2174/1389201043377020
UR  - https://juser.fz-juelich.de/record/38306
ER  -