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@ARTICLE{Gould:38398,
author = {Gould, N. and Thorpe, M. R. and Minchin, P. E. H. and
Pritchard, J. and White, P. J.},
title = {{S}olute is imported to elongating root cells of barley as
a pressure driven flow of solution},
journal = {Functional plant biology},
volume = {31},
issn = {1445-4408},
address = {Collingwood, Victoria},
publisher = {CSIRO Publ.},
reportid = {PreJuSER-38398},
pages = {391 - 397},
year = {2004},
note = {Record converted from VDB: 12.11.2012},
abstract = {This work relates solute import to elongating root cells in
barley to the water relations of the symplastic pathway
under conditions of varied plant K+ status. K+ is a major
constituent of phloem sieve element (SE) sap, and as an
osmoticum, it is believed to have a role in maintaining SE
hydrostatic pressure and thus sap flow from source to sink
tissue. The hypothesis that the solute import to elongating
root cells is linked to pressure driven flow from the sieve
tube is examined.Plants were grown in solutions containing
either 0.05 m M (low K) or 2.05 m M (high K) K+
concentration. Solute import to the root elongation zone was
estimated from biomass accumulation over time accounting for
respiration and root elongation rate. SE sap K+
concentration was measured using X-ray microanalyses and
osmotic pressure by picolitre osmometry. SE hydrostatic
pressure was measured directly with a pressure probe glued
onto an excised aphid stylet. Elongating root cell
hydrostatic pressure was measured using a cell pressure
probe.The low-K plants had lower SE K+ concentration and SE
hydrostatic pressure compared to the high-K plants, but the
elongating root cell hydrostatic pressure was similar in
both treatments, thus the pressure difference between the SE
and elongating root cells was less in the low-K plants
compared to the high-K plants.The solute import rate to
elongating root cells was lower in the low K plants and the
reduction could be accounted for as a pressure driven solute
flux, with a reduction both in the pressure difference
between root sieve elements and elongating cells, and in the
sap concentration.},
keywords = {J (WoSType)},
cin = {ICG-III},
ddc = {580},
cid = {I:(DE-Juel1)VDB49},
pnm = {Chemie und Dynamik der Geo-Biosphäre},
pid = {G:(DE-Juel1)FUEK257},
shelfmark = {Plant Sciences},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000221249800009},
doi = {10.1071/FP03231},
url = {https://juser.fz-juelich.de/record/38398},
}