TY  - JOUR
AU  - Gieß, F.
AU  - Friedrich, M.
AU  - Heberle, J.
AU  - Naumann, R.
AU  - Knoll, W.
TI  - The Protein Tethered Lipid Bilayer - a novel mimic of the biological membrane
JO  - Biophysical journal
VL  - 87
SN  - 0006-3495
CY  - New York, NY
PB  - Rockefeller Univ. Press
M1  - PreJuSER-41694
SP  - 3213 - 3220
PY  - 2004
N1  - Record converted from VDB: 12.11.2012
AB  - A new concept of solid-supported tethered bilayer lipid membrane (tBLM) for the functional incorporation of membrane proteins is introduced. The incorporated protein itself acts as the tethering molecule resulting in a versatile system in which the protein determines the characteristics of the submembraneous space. This architecture is achieved through a metal chelating surface, to which histidine-tagged (His-tagged) membrane proteins are able to bind in a reversible manner. The tethered bilayer lipid membrane is generated by substitution of protein-bound detergent molecules with lipids using in-situ dialysis or adsorption. The system is characterized by surface plasmon resonance, quartz crystal microbalance, and electrochemical impedance spectroscopy. His-tagged cytochrome c oxidase (CcO) is used as a model protein in this study. However, the new system should be applicable to all recombinant membrane proteins bearing a terminal His-tag. In particular, combination of surface immobilization and membrane reconstitution opens new prospects for the investigation of functional membrane proteins by various surface-sensitive techniques under a defined electric field.
KW  - Biomimetics: methods
KW  - Cell Membrane: chemistry
KW  - Electric Impedance
KW  - Electron Transport Complex IV: chemistry
KW  - Histidine: chemistry
KW  - Lipid Bilayers: chemistry
KW  - Macromolecular Substances
KW  - Membrane Fluidity
KW  - Membrane Proteins: chemistry
KW  - Membranes, Artificial
KW  - Molecular Conformation
KW  - Protein Binding
KW  - Lipid Bilayers (NLM Chemicals)
KW  - Macromolecular Substances (NLM Chemicals)
KW  - Membrane Proteins (NLM Chemicals)
KW  - Membranes, Artificial (NLM Chemicals)
KW  - Histidine (NLM Chemicals)
KW  - Electron Transport Complex IV (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:15339795
C2  - pmc:PMC1304791
UR  - <Go to ISI:>//WOS:000224732500026
DO  - DOI:10.1529/biophysj.104.046169
UR  - https://juser.fz-juelich.de/record/41694
ER  -