Journal Article

PreJuSER-41694

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png The Protein Tethered Lipid Bilayer - a novel mimic of the biological membrane

Gieß, F. ; Friedrich, M. ; Heberle, J.FZJ* ; Naumann, R. ; Knoll, W.

2004
Rockefeller Univ. Press New York, NY

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Please use a persistent id in citations: http://hdl.handle.net/2128/700  doi:10.1529/biophysj.104.046169

Abstract: A new concept of solid-supported tethered bilayer lipid membrane (tBLM) for the functional incorporation of membrane proteins is introduced. The incorporated protein itself acts as the tethering molecule resulting in a versatile system in which the protein determines the characteristics of the submembraneous space. This architecture is achieved through a metal chelating surface, to which histidine-tagged (His-tagged) membrane proteins are able to bind in a reversible manner. The tethered bilayer lipid membrane is generated by substitution of protein-bound detergent molecules with lipids using in-situ dialysis or adsorption. The system is characterized by surface plasmon resonance, quartz crystal microbalance, and electrochemical impedance spectroscopy. His-tagged cytochrome c oxidase (CcO) is used as a model protein in this study. However, the new system should be applicable to all recombinant membrane proteins bearing a terminal His-tag. In particular, combination of surface immobilization and membrane reconstitution opens new prospects for the investigation of functional membrane proteins by various surface-sensitive techniques under a defined electric field.

Keyword(s): Biomimetics: methods (MeSH) ; Cell Membrane: chemistry (MeSH) ; Electric Impedance (MeSH) ; Electron Transport Complex IV: chemistry (MeSH) ; Histidine: chemistry (MeSH) ; Lipid Bilayers: chemistry (MeSH) ; Macromolecular Substances (MeSH) ; Membrane Fluidity (MeSH) ; Membrane Proteins: chemistry (MeSH) ; Membranes, Artificial (MeSH) ; Molecular Conformation (MeSH) ; Protein Binding (MeSH) ; Lipid Bilayers ; Macromolecular Substances ; Membrane Proteins ; Membranes, Artificial ; Histidine ; Electron Transport Complex IV ; J

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Biologische Strukturforschung (IBI-2)

Research Program(s):

1. Neurowissenschaften (L01)

Appears in the scientific report 2004
Notes: This version is available at the following Publisher URL: http://www.biophysj.org/

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