%0 Journal Article
%A Pallerla, S. R.
%A Knebel, S.
%A Polen, T.
%A Klauth, P.
%A Hollender, J.
%A Wendisch, V. F.
%A Schoberth, S. M.
%T Formation of Volutin Granules in Corynebacterium glutamicum
%J FEMS microbiology letters
%V 243
%@ 0378-1097
%C Oxford [u.a.]
%I Wiley-Blackwell
%M PreJuSER-41953
%P 133 - 140
%D 2005
%Z Record converted from VDB: 12.11.2012
%X Volutin granules are intracellular storages of complexed inorganic polyphosphate (poly P). Histochemical staining procedures differentiate between pathogenic corynebacteria such as Corynebacterum diphtheriae (containing volutin) and non-pathogenic species, such as C. glutamicum. Here we report that strains ATCC13032 and MH20-22B of the non-pathogenic C. glutamicum also formed subcellular entities (18-37% of the total cell volume) that had the typical characteristics of volutin granules: (i) volutin staining, (ii) green UV fluorescence when stained with 4',6-diamidino-2-phenylindole, (iii) electron-dense and rich in phosphorus when determined with transmission electron microscopy and X-ray microanalysis, and (iv) 31P NMR poly P resonances of isolated granules dissolved in EDTA. MgCl2 addition to the growth medium stimulated granule formation but did not effect expression of genes involved in poly P metabolism. Granular volutin fractions from lysed cells contained polyphosphate glucokinase as detected by SDS-PAGE/MALDI-TOF, indicating that this poly P metabolizing enzyme is present also in intact poly P granules. The results suggest that formation of volutin is a more widespread phenomenon than generally accepted.
%K Bacterial Proteins: genetics
%K Bacterial Proteins: metabolism
%K Corynebacterium glutamicum: genetics
%K Corynebacterium glutamicum: metabolism
%K Corynebacterium glutamicum: ultrastructure
%K Cytoplasmic Granules: metabolism
%K Cytoplasmic Granules: ultrastructure
%K Electron Probe Microanalysis
%K Gene Expression Profiling
%K Gene Expression Regulation, Bacterial
%K Inorganic Chemicals: metabolism
%K Magnesium Chloride: pharmacology
%K Magnetic Resonance Spectroscopy
%K Microscopy, Electron, Transmission
%K Oligonucleotide Array Sequence Analysis
%K Phosphorus Isotopes
%K Phosphotransferases: metabolism
%K Polyphosphates: metabolism
%K Proteins: metabolism
%K Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: methods
%K Bacterial Proteins (NLM Chemicals)
%K Inorganic Chemicals (NLM Chemicals)
%K Phosphorus Isotopes (NLM Chemicals)
%K Polyphosphates (NLM Chemicals)
%K Proteins (NLM Chemicals)
%K Magnesium Chloride (NLM Chemicals)
%K Phosphotransferases (NLM Chemicals)
%K polyphosphate-glucose phosphotransferase (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:15668011
%U <Go to ISI:>//WOS:000226875000019
%R 10.1016/j.femsle.2004.11.047
%U https://juser.fz-juelich.de/record/41953